naa a22 4500 510746519 CHVBK 20180411083048.0 cr unu---uuuuu 180411e20130301xx s 000 0 eng 10.1007/s12192-012-0365-z doi (NATIONALLICENCE)springer-10.1007/s12192-012-0365-z Acute phase proteins are major clients for the chaperone action of α2-macroglobulin in human plasma [Elektronische Daten] [Amy Wyatt, Mark Wilson] Extracellular protein misfolding is implicated in many age-related diseases including Alzheimer's disease, macular degeneration and arthritis. In this study, putative endogenous clients for the chaperone activity of α2-macroglobulin (α2M) were identified after human plasma was subjected to physiologically relevant sheer stress at 37°C for 10days. Western blot analysis showed that four major acute phase proteins: ceruloplasmin, fibrinogen, α1-acid glycoprotein and complement component 3, preferentially co-purified with α2M after plasma was stressed. Furthermore, the formation of complexes between α2M and these putative chaperone clients, detected by sandwich ELISA, was shown to be enhanced in response to stress. These results support the hypothesis that α2M plays an important role in extracellular proteostasis by sequestering misfolded proteins and targeting them for disposal, particularly during acute phase reactions. Cell Stress Society International, 2012 α2-Macroglobulin nationallicence Chaperone nationallicence Protein misfolding nationallicence Acute phase proteins nationallicence Wyatt Amy Department of Chemistry, University of Cambridge, Cambridge, UK aut Wilson Mark Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, NSW, Australia aut Cell Stress and Chaperones Springer Netherlands 18/2(2013-03-01), 161-170 1355-8145 18:2<161 2013 18 12192 https://doi.org/10.1007/s12192-012-0365-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-012-0365-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wyatt Amy Department of Chemistry, University of Cambridge, Cambridge, UK aut NATIONALLICENCE 700 1- Wilson Mark Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, NSW, Australia aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/2(2013-03-01), 161-170 1355-8145 18:2<161 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer