naa a22 4500 510746527 CHVBK 20180411083048.0 cr unu---uuuuu 180411e20130301xx s 000 0 eng 10.1007/s12192-012-0363-1 doi (NATIONALLICENCE)springer-10.1007/s12192-012-0363-1 Hsps are up-regulated in melanoma tissue and correlate with patient clinical parameters [Elektronische Daten] [Christopher Shipp, Benjamin Weide, Evelyna Derhovanessian, Graham Pawelec] Heat shock proteins (hsps) have been studied in numerous cancer types, but a clear view of their clinical relevance in melanoma remains elusive. Therefore, the aim of this study was to investigate the expression of hsps in melanoma with respect to patient clinical parameters. Using Western immunoblotting, hsps 90, 70, 60, 40 and 32 were observed to be widely expressed in metastatic melanomas (n = 31), while immunofluorescence demonstrated that in the majority of samples these hsps, apart from hsp32, were increased in expression in melanoma cells compared with surrounding non-melanoma cells in situ (n = 8). Correlating hsp expression with patient clinical parameters indicated that greater hsp90 (P < 0.02) and hsp40 (P < 0.03) expression correlated with advanced stage (stage III Vs stage IV), while in the case of hsp40, this was additionally associated with reduced patient survival (P < 0.05). In contrast, higher hsp32 expression was associated with improved patient survival (P < 0.007). On the other hand, the expression of the other hsps did not correlate with any obtainable patient clinical parameters. This study provides further evidence for the importance of hsps in melanoma and for their use as therapeutic targets and biomarkers, but larger-scale follow-up studies are required to confirm these results. Cell Stress Society International, 2012 Metastatic melanoma nationallicence Heat shock proteins nationallicence Hsps nationallicence Patient clinical parameters nationallicence Western immunoblotting nationallicence Immunofluorescence nationallicence Shipp Christopher Tübingen Ageing and Tumour Immunology Group, Section for Transplantation Immunology, Department of Internal Medicine II, Centre for Medical Research, University of Tübingen, Waldhörnlestr. 22, 72072, Tübingen, Germany aut Weide Benjamin Division of Dermatooncology, Department of Dermatology, University Medical Center, Tübingen, Germany aut Derhovanessian Evelyna Tübingen Ageing and Tumour Immunology Group, Section for Transplantation Immunology, Department of Internal Medicine II, Centre for Medical Research, University of Tübingen, Waldhörnlestr. 22, 72072, Tübingen, Germany aut Pawelec Graham Tübingen Ageing and Tumour Immunology Group, Section for Transplantation Immunology, Department of Internal Medicine II, Centre for Medical Research, University of Tübingen, Waldhörnlestr. 22, 72072, Tübingen, Germany aut Cell Stress and Chaperones Springer Netherlands 18/2(2013-03-01), 145-154 1355-8145 18:2<145 2013 18 12192 https://doi.org/10.1007/s12192-012-0363-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12192-012-0363-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shipp Christopher Tübingen Ageing and Tumour Immunology Group, Section for Transplantation Immunology, Department of Internal Medicine II, Centre for Medical Research, University of Tübingen, Waldhörnlestr. 22, 72072, Tübingen, Germany aut NATIONALLICENCE 700 1- Weide Benjamin Division of Dermatooncology, Department of Dermatology, University Medical Center, Tübingen, Germany aut NATIONALLICENCE 700 1- Derhovanessian Evelyna Tübingen Ageing and Tumour Immunology Group, Section for Transplantation Immunology, Department of Internal Medicine II, Centre for Medical Research, University of Tübingen, Waldhörnlestr. 22, 72072, Tübingen, Germany aut NATIONALLICENCE 700 1- Pawelec Graham Tübingen Ageing and Tumour Immunology Group, Section for Transplantation Immunology, Department of Internal Medicine II, Centre for Medical Research, University of Tübingen, Waldhörnlestr. 22, 72072, Tübingen, Germany aut NATIONALLICENCE 773 0- Cell Stress and Chaperones Springer Netherlands 18/2(2013-03-01), 145-154 1355-8145 18:2<145 2013 18 12192 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer