naa a22 4500 51074656X CHVBK 20180411083048.0 cr unu---uuuuu 180411e20130901xx s 000 0 eng 10.1007/s13199-013-0254-y doi (NATIONALLICENCE)springer-10.1007/s13199-013-0254-y Effects of the symbiosis between fungal endophytes and Atractylodes lancea on rhizosphere and phyllosphere microbial communities [Elektronische Daten] [Teng Yang, Wei Du, Jun Zhou, Xing-Xiang Wang, Chuan-Chao Dai] Tissue-cultured plantlets of Atractylodes lancea were inoculated with the endophytes AL4 (Cunninghamella sp.) and AL12 (Gilmaniella sp.), and subsequently transplanted into soil after hardening of the tissue-cultured plantlets. We investigated rhizospheric and phyllospheric microbial communities using culture-based and culture-independent methods. Energy spectrum analysis, high performance liquid chromatography, and other assay methods were employed to quantify the elements in the leaves, and the soluble sugars, free amino acids and organic acids in the rhizosphere. The results showed that the endophytes enhanced the diversity and size of the rhizospheric microbial populations. In the phyllosphere, AL4 (Cunninghamella sp.) enhanced the diversity and size of bacterial populations, while AL12 (Gilmaniella sp.) enhanced the diversity and size of fungal populations. The dominant bacterial genera were Microbacterium, Kocuria and Sphingomon in the endophytes-inoculated groups, and Acinetobacter and Bacillus in the endophytes-free group. While Acremonium and Curvularia were the dominant fungal genera in the phyllosphere of endophytes-inoculated groups, Fusarium and Penicillum were most common in the endophytes-free group. AL4 (Cunninghamella sp.) enhanced the rhizospheric microbial population size and diversity by increasing rhizospheric free amino acids, while AL12 (Gilmaniella sp.) altered the rhizospheric microbes by changing concentration of soluble sugars in the rhizosphere. Elemental levels in the phyllosphere and the nutrients in the rhizosphere varied among the treatments and may also have influenced the microbial communities. Springer Science+Business Media Dordrecht, 2013 Fungal endophytes nationallicence Atractylodes lancea nationallicence Leaf leachates nationallicence Root exudates nationallicence Microbial communities nationallicence PCR-DGGE : Polymerase chain reaction-denaturing gradient gel electrophoresis nationallicence HPLC : High performance liquid chromatography nationallicence DAP : 4′,6-Diamidino-2-phenylindole nationallicence SEM : Scanning electron microscope nationallicence QS : Quorum sensing nationallicence Yang Teng Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, 210023, Nanjing, People's Republic of China aut Du Wei Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, 210023, Nanjing, People's Republic of China aut Zhou Jun Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, 210023, Nanjing, People's Republic of China aut Wang Xing-Xiang Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, People's Republic of China aut Dai Chuan-Chao Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, 210023, Nanjing, People's Republic of China aut Symbiosis Springer Netherlands 61/1(2013-09-01), 23-36 0334-5114 61:1<23 2013 61 13199 https://doi.org/10.1007/s13199-013-0254-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13199-013-0254-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Teng Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, 210023, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Du Wei Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, 210023, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Jun Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, 210023, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Wang Xing-Xiang Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Dai Chuan-Chao Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, 210023, Nanjing, People's Republic of China aut NATIONALLICENCE 773 0- Symbiosis Springer Netherlands 61/1(2013-09-01), 23-36 0334-5114 61:1<23 2013 61 13199 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer