naa a22 4500 510746578 CHVBK 20180411083048.0 cr unu---uuuuu 180411e20130901xx s 000 0 eng 10.1007/s13199-013-0256-9 doi (NATIONALLICENCE)springer-10.1007/s13199-013-0256-9 Two endosymbiotic bacteria, Wolbachia and Arsenophonus , in the brown planthopper Nilaparvata lugens [Elektronische Daten] [Lv-Yu Qu, Yi-Han Lou, Hai-Wei Fan, Yu-Xuan Ye, Hai-Jian Huang, Meng-Qi Hu, Ya-Ni Zhu, Chuan-Xi Zhang] The brown planthopper Nilaparvata lugens harbors intracellular fungal yeast-like symbionts and endosymbiotic bacteria, with the latter mainly comprising Wolbachia and Arsenophonus. In this study, Wolbachia or Arsenophonus were detected in all 15 brown planthopper populations collected from China and Southeastern Asian countries. Furthermore, Polymerase Chain Reaction (PCR) analysis of the individuals in a population that was infected by both Wolbachia and Arsenophonus showed that each individual was infected by only one of the two symbiotic bacteria. Real-time quantitative PCR showed that both endosymbionts are mainly localized in the mycetocytes of the fat body. Reciprocal crosses between the Wolbachia + and Arsenophonus + brown planthopper populations showed that both bacteria were maternally transmitted. Our results showed that the brown planthopper populations are extensively infected by Wolbachia or Arsenophonus, and the two bacteria may be exclusive in each host individual. This finding might be helpful for further studies on the biological functions of the endosymbiotic bacteria and will deepen our understanding of the complicated symbiosis system in this host. Springer Science+Business Media Dordrecht, 2013 Wolbachia nationallicence Arsenophonus nationallicence Endosymbionts nationallicence Exclusive relationship nationallicence Nilaparvata lugens nationallicence Qu Lv-Yu Institute of Insect Science, Zhejiang University, Yuhangtang Road 866, Hangzhou, China aut Lou Yi-Han Institute of Insect Science, Zhejiang University, Yuhangtang Road 866, Hangzhou, China aut Fan Hai-Wei Institute of Insect Science, Zhejiang University, Yuhangtang Road 866, Hangzhou, China aut Ye Yu-Xuan Institute of Insect Science, Zhejiang University, Yuhangtang Road 866, Hangzhou, China aut Huang Hai-Jian Institute of Insect Science, Zhejiang University, Yuhangtang Road 866, Hangzhou, China aut Hu Meng-Qi Institute of Insect Science, Zhejiang University, Yuhangtang Road 866, Hangzhou, China aut Zhu Ya-Ni Institute of Insect Science, Zhejiang University, Yuhangtang Road 866, Hangzhou, China aut Zhang Chuan-Xi Institute of Insect Science, Zhejiang University, Yuhangtang Road 866, Hangzhou, China aut Symbiosis Springer Netherlands 61/1(2013-09-01), 47-53 0334-5114 61:1<47 2013 61 13199 https://doi.org/10.1007/s13199-013-0256-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13199-013-0256-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Qu Lv-Yu Institute of Insect Science, Zhejiang University, Yuhangtang Road 866, Hangzhou, China aut NATIONALLICENCE 700 1- Lou Yi-Han Institute of Insect Science, Zhejiang University, Yuhangtang Road 866, Hangzhou, China aut NATIONALLICENCE 700 1- Fan Hai-Wei Institute of Insect Science, Zhejiang University, Yuhangtang Road 866, Hangzhou, China aut NATIONALLICENCE 700 1- Ye Yu-Xuan Institute of Insect Science, Zhejiang University, Yuhangtang Road 866, Hangzhou, China aut NATIONALLICENCE 700 1- Huang Hai-Jian Institute of Insect Science, Zhejiang University, Yuhangtang Road 866, Hangzhou, China aut NATIONALLICENCE 700 1- Hu Meng-Qi Institute of Insect Science, Zhejiang University, Yuhangtang Road 866, Hangzhou, China aut NATIONALLICENCE 700 1- Zhu Ya-Ni Institute of Insect Science, Zhejiang University, Yuhangtang Road 866, Hangzhou, China aut NATIONALLICENCE 700 1- Zhang Chuan-Xi Institute of Insect Science, Zhejiang University, Yuhangtang Road 866, Hangzhou, China aut NATIONALLICENCE 773 0- Symbiosis Springer Netherlands 61/1(2013-09-01), 47-53 0334-5114 61:1<47 2013 61 13199 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer