naa a22 4500 510746594 CHVBK 20180411083048.0 cr unu---uuuuu 180411e20131101xx s 000 0 eng 10.1007/s13199-013-0266-7 doi (NATIONALLICENCE)springer-10.1007/s13199-013-0266-7 Effect of insect host age and diet on the fitness of the entomopathogenic nematode-bacteria mutualism [Elektronische Daten] [Victoria Miranda, Patricia Navarro, Goggy Davidowitz, Judith Bronstein, S. Stock] Insect host age and diet were evaluated as potential factors that could affect the fitness of the entomopathogenic nematode-bacterium mutualistic partnership. Two nematode species were considered: Steinernema carpocapsae and Heterorhabditis sonorensis, together with their symbionts Xenorhabdus nematophila and Photorhabdus luminescens, respectively. The tobacco hornworm, Manduca sexta, was used as the insect host. Insect developmental stage was a factor that impacted nematode virulence. Non-wandering 5th instar M. sexta were found to be more susceptible to nematode infection compared to wandering 5th instars. This was more noticeable for S. carpocapsae than for H. sonorensis. The nutritional status of the host also had an effect on the fitness of the two nematode species tested. In general, insects fed with the reduced diet content were less susceptible to nematode parasitism. The least observed mortality (0.5%) was in those M. sexta larvae exposed to the low H. sonorensis dose. Host diet also had an effect on the production of IJ progeny in the insect cadavers. For both nematode species tested, the highest yield of emerging IJs was observed from those insect hosts fed with the low nutrient diet and exposed to the highest nematode inoculum. However, for both nematode species tested, the nutritional status of the host did not significantly affect time of emergence of IJ progeny or the reassociation with their bacterial symbionts (expressed as cfu/IJ). This is the first study on the effect of insect host physiology on both EPN and their symbiotic bacteria fitness. Springer Science+Business Media Dordrecht, 2013 Manduca sexta nationallicence Steinernema carpocapsae nationallicence Xenorhabdus nematophila nationallicence Heterorhabditis sonorensis nationallicence Photorhabdus luminescens nationallicence Host diet nationallicence Host age nationallicence Miranda Victoria Department of Entomology, University of Arizona, 85721, Tucson, AZ, USA aut Navarro Patricia Institituto Nacional de Tecnología Agraria, INIA Carillanca, Camino Cajón-Vilcún km.10, Vilcún, Chile aut Davidowitz Goggy Department of Entomology, University of Arizona, 85721, Tucson, AZ, USA aut Bronstein Judith Department of Entomology, University of Arizona, 85721, Tucson, AZ, USA aut Stock S. Department of Entomology, University of Arizona, 85721, Tucson, AZ, USA aut Symbiosis Springer Netherlands 61/3(2013-11-01), 145-153 0334-5114 61:3<145 2013 61 13199 https://doi.org/10.1007/s13199-013-0266-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13199-013-0266-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miranda Victoria Department of Entomology, University of Arizona, 85721, Tucson, AZ, USA aut NATIONALLICENCE 700 1- Navarro Patricia Institituto Nacional de Tecnología Agraria, INIA Carillanca, Camino Cajón-Vilcún km.10, Vilcún, Chile aut NATIONALLICENCE 700 1- Davidowitz Goggy Department of Entomology, University of Arizona, 85721, Tucson, AZ, USA aut NATIONALLICENCE 700 1- Bronstein Judith Department of Entomology, University of Arizona, 85721, Tucson, AZ, USA aut NATIONALLICENCE 700 1- Stock S. Department of Entomology, University of Arizona, 85721, Tucson, AZ, USA aut NATIONALLICENCE 773 0- Symbiosis Springer Netherlands 61/3(2013-11-01), 145-153 0334-5114 61:3<145 2013 61 13199 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer