naa a22 4500 510746608 CHVBK 20180411083048.0 cr unu---uuuuu 180411e20131101xx s 000 0 eng 10.1007/s13199-013-0264-9 doi (NATIONALLICENCE)springer-10.1007/s13199-013-0264-9 Identification of genes involved in salt tolerance and symbiotic nitrogen fixation in chickpea rhizobium Mesorhizobium ciceri Ca181 [Elektronische Daten] [Tripti Dogra, Anupama Priyadarshini, Kanika,, Ashok Kumar, Nagendra Singh] In an attempt to find the genes involved in salt tolerance of the highly adaptable chickpea rhizobium strain, Mesorhizobium ciceri Ca181, a Tn5 transposon insertion library was generated and screened to identify five mutants with inability to survive in the presence of 0.1M NaCl. The genes disrupted in these mutants due to insertion of the transposon were identified by sequencing of Tn5 flanking sequences after inverse PCR. One of the mutants had a disruption in diguanylate cyclase gene which is involved in bacterial biofilm formation and persistence. The second mutant had a disruption in an ABC transporter membrane protein gene, which is involved in the uptake of nutrients and cellular osmoprotection. The third mutant had a disruption in a gene showing homology with rhamnulose 1-phosphate aldolase which has an important role in the central metabolism of L-rhamnulose. The fourth mutant had a disruption in a capsule synthesis gene and the fifth mutant had an insertion in an oxidoreductase gene. When these mutants were inoculated into the host chickpea plant under normal non-saline conditions, they formed symbiotic nodules but with severely reduced nitrogenase activity. Hence, it appears that bacterial ability to adapt to hyper-osmotic salt stress conditions is also important for its nitrogen fixing ability in the chickpea root nodules. Allele mining for variant forms of the identified genes in the germplasm resources of M. ciceri may help in the development of highly adaptive and efficient nitrogen fixing strains of the chickpea rhizobium. Springer Science+Business Media Dordrecht, 2013 Mesorhizobium ciceri nationallicence Mutant library nationallicence Salt tolerance nationallicence Tn5 mutagenesis nationallicence Transposome nationallicence Dogra Tripti National Research Centre on Plant Biotechnology, IARI Campus, New Delhi 12, India aut Priyadarshini Anupama National Research Centre on Plant Biotechnology, IARI Campus, New Delhi 12, India aut Kanika, National Research Centre on Plant Biotechnology, IARI Campus, New Delhi 12, India aut Kumar Ashok Banaras Hindu University, Varanasi, Uttar Pradesh, India aut Singh Nagendra National Research Centre on Plant Biotechnology, IARI Campus, New Delhi 12, India aut Symbiosis Springer Netherlands 61/3(2013-11-01), 135-143 0334-5114 61:3<135 2013 61 13199 https://doi.org/10.1007/s13199-013-0264-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13199-013-0264-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dogra Tripti National Research Centre on Plant Biotechnology, IARI Campus, New Delhi 12, India aut NATIONALLICENCE 700 1- Priyadarshini Anupama National Research Centre on Plant Biotechnology, IARI Campus, New Delhi 12, India aut NATIONALLICENCE 700 1- Kanika National Research Centre on Plant Biotechnology, IARI Campus, New Delhi 12, India aut NATIONALLICENCE 700 1- Kumar Ashok Banaras Hindu University, Varanasi, Uttar Pradesh, India aut NATIONALLICENCE 700 1- Singh Nagendra National Research Centre on Plant Biotechnology, IARI Campus, New Delhi 12, India aut NATIONALLICENCE 773 0- Symbiosis Springer Netherlands 61/3(2013-11-01), 135-143 0334-5114 61:3<135 2013 61 13199 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer