naa a22 4500 510746624 CHVBK 20180411083048.0 cr unu---uuuuu 180411e20131101xx s 000 0 eng 10.1007/s13199-013-0262-y doi (NATIONALLICENCE)springer-10.1007/s13199-013-0262-y Real time PCR detection targeting nifA gene of plant growth promoting bacteria Azospirillum brasilense strain FP2 in maize roots [Elektronische Daten] [Alexandro Faleiro, Tomás Pereira, Eliandro Espindula, Fábio Brod, Ana Arisi] The plant growth promoting bacteria (PGPB) Azospirillum brasilense has been recommended for use in commercial inoculants in Brazil. Effective methods are necessary to monitor PGPB strains in the rhizosphere. Our purpose was to develop a real time PCR method for detection of A. brasilense strain FP2 in maize seedlings targeting nifA. Primer pairs were designed and their specificity was verified using DNA from 12 different bacterial species. Standard curves were prepared for DNA quantification using serial dilution of A. brasilense DNA extracts. PCR efficiencies and correlation coefficient presented values within the acceptable range for qPCR, mean PCR efficiency was 95% and correlation coefficient was 0.98, indicating that nifA gene was suitable for the quantitative analysis of the target bacterial genome. Inoculated maize seedlings were grown in vitro or in pots, bacterial DNA copy number per gram of fresh root was quantified 1, 4, 7 and 10days after inoculation. The developed primers targeting nifA will be useful for monitoring Azospirillum brasilense strain FP2 in crops. Springer Science+Business Media Dordrecht, 2013 Azospirillum brasilense nationallicence Plant-bacteria interaction nationallicence Maize nationallicence qPCR nationallicence Inoculant nationallicence CFU : Colony forming units nationallicence Ct : Cycle threshold nationallicence CTAB : Cetyltrimethylammonium bromide nationallicence CV : Coefficient of variation nationallicence DAI : Days after inoculation nationallicence PCR : Polymerase chain reaction nationallicence qPCR : Quantitative polymerase chain reaction nationallicence SD : Standard deviation nationallicence Faleiro Alexandro Departamento de Ciência e Tecnologia de Alimentos, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Rod. Admar Gonzaga, 1346, 88034-001, Florianópolis, SC, Brazil aut Pereira Tomás Departamento de Ciência e Tecnologia de Alimentos, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Rod. Admar Gonzaga, 1346, 88034-001, Florianópolis, SC, Brazil aut Espindula Eliandro Departamento de Ciência e Tecnologia de Alimentos, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Rod. Admar Gonzaga, 1346, 88034-001, Florianópolis, SC, Brazil aut Brod Fábio Departamento de Ciência e Tecnologia de Alimentos, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Rod. Admar Gonzaga, 1346, 88034-001, Florianópolis, SC, Brazil aut Arisi Ana Departamento de Ciência e Tecnologia de Alimentos, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Rod. Admar Gonzaga, 1346, 88034-001, Florianópolis, SC, Brazil aut Symbiosis Springer Netherlands 61/3(2013-11-01), 125-133 0334-5114 61:3<125 2013 61 13199 https://doi.org/10.1007/s13199-013-0262-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13199-013-0262-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Faleiro Alexandro Departamento de Ciência e Tecnologia de Alimentos, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Rod. Admar Gonzaga, 1346, 88034-001, Florianópolis, SC, Brazil aut NATIONALLICENCE 700 1- Pereira Tomás Departamento de Ciência e Tecnologia de Alimentos, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Rod. Admar Gonzaga, 1346, 88034-001, Florianópolis, SC, Brazil aut NATIONALLICENCE 700 1- Espindula Eliandro Departamento de Ciência e Tecnologia de Alimentos, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Rod. Admar Gonzaga, 1346, 88034-001, Florianópolis, SC, Brazil aut NATIONALLICENCE 700 1- Brod Fábio Departamento de Ciência e Tecnologia de Alimentos, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Rod. Admar Gonzaga, 1346, 88034-001, Florianópolis, SC, Brazil aut NATIONALLICENCE 700 1- Arisi Ana Departamento de Ciência e Tecnologia de Alimentos, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Rod. Admar Gonzaga, 1346, 88034-001, Florianópolis, SC, Brazil aut NATIONALLICENCE 773 0- Symbiosis Springer Netherlands 61/3(2013-11-01), 125-133 0334-5114 61:3<125 2013 61 13199 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer