naa a22 4500 510746667 CHVBK 20180411083048.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s13199-013-0258-7 doi (NATIONALLICENCE)springer-10.1007/s13199-013-0258-7 Application of nonspecific commercial AMF inocula results in poor mycorrhization in Camellia japonica L [Elektronische Daten] [Andrea Berruti, Roberto Borriello, Maria Della Beffa, Valentina Scariot, Valeria Bianciotto] Camellia japonica L. (Theaceae, Theales) is an acidophilic evergreen flowering shrub and is traded worldwide. Symbiotic associations between the roots of this plant species and arbuscular mycorrhizal fungi (AMF), commonly recognized as natural biofertilizers and biocontrol agents, have been poorly studied so far. The aim of our study was to verify whether the application of nonspecific commercial AMF-based inocula could succeed and improve the growth of C. japonica. An experiment was conducted concerning the application of commercial inocula constituted by a specific AMF isolate (Funneliformis mosseae) or a consortium of different fungi and bacteria as alternatives to fertilization in pot cultivated C. japonica ‘Dr. Burnside'. Several growth parameters, plant nutrition and mycorrhization levels were monitored at the end of plant cultivation. Generally, increases in some macroelements (Ca, Mg, K) and microelements (Cu, Mn, Fe, Zn) were detected in the root system of inoculated camellias, while only Cu and Mn accumulation was increased in the leaves. Plants inoculated with the consortium inoculum exhibited increased chlorophyll content and foliage diameter while plants inoculated with F. mosseae exhibited increased number of flowers. Based on the polymorphism of an 18S rDNA region, we assessed the inoculated AMF that colonized C. japonica roots. The experiments showed that the applied AMF poorly colonized the root system of C. japonica. We suggest that commercial AMF formulations should be more targeted and host-specific in order to successfully colonize the host root and, potentially, fully express their benefits. Springer Science+Business Media Dordrecht, 2013 Arbuscular mycorrhizal fungi nationallicence Biofertilizer inoculation nationallicence Microbial consortium nationallicence 18S rDNA nationallicence Sustainable floriculture nationallicence Berruti Andrea Department of Agricultural, Forest and Food Sciences, University of Torino, via Leonardo da Vinci 44, 10095, Grugliasco, TO, Italy aut Borriello Roberto National Research Council (CNR), Plant Protection Institute - Turin UOS, viale Mattioli 25, 10125, Torino, Italy aut Della Beffa Maria National Research Council (CNR), Plant Protection Institute - Turin UOS, viale Mattioli 25, 10125, Torino, Italy aut Scariot Valentina Department of Agricultural, Forest and Food Sciences, University of Torino, via Leonardo da Vinci 44, 10095, Grugliasco, TO, Italy aut Bianciotto Valeria National Research Council (CNR), Plant Protection Institute - Turin UOS, viale Mattioli 25, 10125, Torino, Italy aut Symbiosis Springer Netherlands 61/2(2013-10-01), 63-76 0334-5114 61:2<63 2013 61 13199 https://doi.org/10.1007/s13199-013-0258-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13199-013-0258-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Berruti Andrea Department of Agricultural, Forest and Food Sciences, University of Torino, via Leonardo da Vinci 44, 10095, Grugliasco, TO, Italy aut NATIONALLICENCE 700 1- Borriello Roberto National Research Council (CNR), Plant Protection Institute - Turin UOS, viale Mattioli 25, 10125, Torino, Italy aut NATIONALLICENCE 700 1- Della Beffa Maria National Research Council (CNR), Plant Protection Institute - Turin UOS, viale Mattioli 25, 10125, Torino, Italy aut NATIONALLICENCE 700 1- Scariot Valentina Department of Agricultural, Forest and Food Sciences, University of Torino, via Leonardo da Vinci 44, 10095, Grugliasco, TO, Italy aut NATIONALLICENCE 700 1- Bianciotto Valeria National Research Council (CNR), Plant Protection Institute - Turin UOS, viale Mattioli 25, 10125, Torino, Italy aut NATIONALLICENCE 773 0- Symbiosis Springer Netherlands 61/2(2013-10-01), 63-76 0334-5114 61:2<63 2013 61 13199 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer