naa a22 4500 510746675 CHVBK 20180411083048.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s13199-013-0257-8 doi (NATIONALLICENCE)springer-10.1007/s13199-013-0257-8 Primary symbiont of the ancient scale insect family Coelostomidiidae exhibits strict cophylogenetic patterns [Elektronische Daten] [Manpreet Dhami, Thomas Buckley, Jacqueline Beggs, Michael Taylor] Bacterial symbionts play a critical role in the physiology, ecology and evolution of a diverse range of insects. Such symbionts with unknown roles in the ecology and evolution of their hosts have been reported from archaeococcoid scale insects of family Coelostomidiidae. We examine in detail the bacterial community associated with the remaining species of this family, and calculate the cophylogenetic relationship between the hosts and their symbionts. The 28S ribosomal RNA (rRNA) and mitochondrial cytochrome oxidase I genes were used to reconstruct the host phylogeny while the 16S rRNA gene was used for the bacterial phylogeny. Three well-supported clades were detected within the phylogeny of the monophyletic family Coelostomidiidae. Besides the known symbionts, a novel Sodalis-like symbiont was detected from three of the species. The primary bacteriome inhabiting B-symbiont (Bacteroidetes; ‘Candidatus Hoataupuhia coelostomidicola') was widespread across the host family. Cophylogenetic comparison using Jungles-based reconciliation analysis and ParaFit statistical test revealed a strongly congruent phylogeny of this symbiont with the host family, with no host-switches and few losses and duplications. A similar pattern was observed across a relatively unrelated neococcoid family that exhibits a different physiology and symbiont community, besides a related Bacteroidetes symbiont. We reconfirm that the B-symbiont is a primary symbiont, owing to its strongly congruent evolution with the host and its bacteriome-inhabiting nature. Our analysis affirms recent suggestions that the Bacteroidetes-affiliated symbionts may have driven the hyper-diversification of scale insects worldwide. Springer Science+Business Media Dordrecht, 2013 Intracellular bacteria nationallicence Symbiosis nationallicence New Zealand nationallicence Bacteroidetes nationallicence Phylogeny reconciliation nationallicence Dhami Manpreet Centre for Microbial Innovation, The University of Auckland, Auckland Mail Centre, Private Bag 92019, 1142, Auckland, New Zealand aut Buckley Thomas Landcare Research, Private Bag 92170, Auckland, New Zealand aut Beggs Jacqueline The School of Biological Sciences, The University of Auckland, Auckland, New Zealand aut Taylor Michael Centre for Microbial Innovation, The University of Auckland, Auckland Mail Centre, Private Bag 92019, 1142, Auckland, New Zealand aut Symbiosis Springer Netherlands 61/2(2013-10-01), 77-91 0334-5114 61:2<77 2013 61 13199 https://doi.org/10.1007/s13199-013-0257-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13199-013-0257-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dhami Manpreet Centre for Microbial Innovation, The University of Auckland, Auckland Mail Centre, Private Bag 92019, 1142, Auckland, New Zealand aut NATIONALLICENCE 700 1- Buckley Thomas Landcare Research, Private Bag 92170, Auckland, New Zealand aut NATIONALLICENCE 700 1- Beggs Jacqueline The School of Biological Sciences, The University of Auckland, Auckland, New Zealand aut NATIONALLICENCE 700 1- Taylor Michael Centre for Microbial Innovation, The University of Auckland, Auckland Mail Centre, Private Bag 92019, 1142, Auckland, New Zealand aut NATIONALLICENCE 773 0- Symbiosis Springer Netherlands 61/2(2013-10-01), 77-91 0334-5114 61:2<77 2013 61 13199 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer