naa a22 4500 510746748 CHVBK 20180411083049.0 cr unu---uuuuu 180411e20130301xx s 000 0 eng 10.1007/s13199-013-0223-5 doi (NATIONALLICENCE)springer-10.1007/s13199-013-0223-5 Japanese barberry seed predation by Rhagoletis meigenii fruit flies harboring Wolbachia endosymbionts [Elektronische Daten] [Vanessa Coats, Philip Stack, Mary Rumpho] The phytophagous fruit fly Rhagoletis meigenii harbors the bacterium Wolbachia pipientis and, together with Japanese barberry, form a tri-partite symbiosis. R. meigenii is a seed predator of invasive Japanese barberry plants and is dependent on this insect-plant interaction for reproductive success. The endosymbiotic bacterium W. pipientis is a reproductive parasite known to alter the sex ratios of offspring and the fitness of infected host insects. We investigated Japanese barberry fruit for the degree of infestation by R. meigenii and characterized the Wolbachia strain infecting R. meigenii. Densities of R. meigenii in four naturalized stands of Japanese barberry revealed low numbers of fruit flies with high variability in the population densities observed among individual plants. Overall, R. meigenii infested roughly 10-20% of the Japanese barberry fruits analyzed; fruit with two seeds (vs. one seed) were the most frequently infested. Approximately, 90% of the R. meigenii tested positive for Wolbachia infection via PCR amplification of the Wolbachia surface protein (wsp) gene. No bacterial strain diversity was observed when comparing multi-locus sequence typing (MLST) profiles within or among five R. meigenii populations in Maine, although the MLST profile obtained from R. meigenii differed from three co-occurring Rhagoletis. The Wolbachia endosymbiont of R. meigenii is a member of the Wolbachia supergroup A and the ST-13 cluster complex. Springer Science+Business Media Dordrecht, 2013 Japanese barberry nationallicence Invasive plant nationallicence Rhagoletis meigenii nationallicence Phytophagy nationallicence Predisperal seed predation nationallicence Plant-insect interaction nationallicence Wolbachia pipientis nationallicence Bacteria symbiosis nationallicence Coats Vanessa Department of Molecular and Biomedical Sciences, University of Maine, 5735 Hitchner Hall, 04469, Orono, ME, USA aut Stack Philip School of Biology and Ecology, University of Maine, 5751 Murray Hall, 04461, Orono, ME, USA aut Rumpho Mary Department of Molecular and Biomedical Sciences, University of Maine, 5735 Hitchner Hall, 04469, Orono, ME, USA aut Symbiosis Springer Netherlands 59/3(2013-03-01), 145-156 0334-5114 59:3<145 2013 59 13199 https://doi.org/10.1007/s13199-013-0223-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13199-013-0223-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Coats Vanessa Department of Molecular and Biomedical Sciences, University of Maine, 5735 Hitchner Hall, 04469, Orono, ME, USA aut NATIONALLICENCE 700 1- Stack Philip School of Biology and Ecology, University of Maine, 5751 Murray Hall, 04461, Orono, ME, USA aut NATIONALLICENCE 700 1- Rumpho Mary Department of Molecular and Biomedical Sciences, University of Maine, 5735 Hitchner Hall, 04469, Orono, ME, USA aut NATIONALLICENCE 773 0- Symbiosis Springer Netherlands 59/3(2013-03-01), 145-156 0334-5114 59:3<145 2013 59 13199 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer