naa a22 4500 510746764 CHVBK 20180411083049.0 cr unu---uuuuu 180411e20130301xx s 000 0 eng 10.1007/s13199-013-0228-0 doi (NATIONALLICENCE)springer-10.1007/s13199-013-0228-0 Twenty-five cultures of lichenizing fungi available for experimental studies on symbiotic systems [Elektronische Daten] [Tami McDonald, Ester Gaya, François Lutzoni] In this study we describe the techniques used to culture 25 mycobionts spanning three classes and five orders of the leotiomyceta (Ascomycota). We find that five media, including potato-carrot, malt extract-yeast extract (MY), Bold's basal medium with nitrogen (NMBBM), oatmeal, and yeast extract with supplements (YES), are sufficient to induce ascospore germination of many lichenizing fungi and are also suitable for maintaining growth of the culture over the long term. Regular physical disruption of the cultures in liquid media is recommended to stimulate continued growth. Genomes of five of these lichen-forming fungal strains have been sequenced. The identity of each culture was confirmed by sequencing the nuclear ribosomal internal transcribed spacer (ITS) or the mitochondrial small subunit (mitSSU) from each strain. Additionally, the level of sequencing in terms of total number of genes sequenced for each taxon is provided. All fungal cultures have been deposited in public culture collections and, therefore, are available to the scientific community for conducting in vitro experiments. Springer Science+Business Media Dordrecht, 2013 Culture nationallicence Lichens nationallicence Fungi nationallicence McDonald Tami Department of Microbiology, University of Minnesota, MMC 196 420 Delaware St. SE, 55455, Minneapolis, MN, USA aut Gaya Ester Biology Department, Duke University, Durham, NC, USA aut Lutzoni François Biology Department, Duke University, Durham, NC, USA aut Symbiosis Springer Netherlands 59/3(2013-03-01), 165-171 0334-5114 59:3<165 2013 59 13199 https://doi.org/10.1007/s13199-013-0228-0 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13199-013-0228-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- McDonald Tami Department of Microbiology, University of Minnesota, MMC 196 420 Delaware St. SE, 55455, Minneapolis, MN, USA aut NATIONALLICENCE 700 1- Gaya Ester Biology Department, Duke University, Durham, NC, USA aut NATIONALLICENCE 700 1- Lutzoni François Biology Department, Duke University, Durham, NC, USA aut NATIONALLICENCE 773 0- Symbiosis Springer Netherlands 59/3(2013-03-01), 165-171 0334-5114 59:3<165 2013 59 13199 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer