naa a22 4500 510746772 CHVBK 20180411083049.0 cr unu---uuuuu 180411e20130301xx s 000 0 eng 10.1007/s13199-013-0229-z doi (NATIONALLICENCE)springer-10.1007/s13199-013-0229-z Two effects of combined nitrogen on the adhesion of Rhizobium etli to bean roots [Elektronische Daten] [Julieta Pérez-Giménez, Aníbal Lodeiro] Combined forms of nitrogen negatively influence rhizobia-legume symbiosis. The effects of combined nitrogen are known for nodulation and dinitrogen (N2) fixation, but little is known about the effect on preinfection events. Here, we studied the effects of combined nitrogen on the adhesion of Rhizobium etli to common bean (Phaseolus vulgaris L.) roots. When potassium nitrate (KNO3) or sodium glutamate was added to an incubation mixture of rhizobia and plants that were previously grown in nitrogen-free solution, rhizobial adhesion to roots was stimulated. However, the rhizobial adhesion to bean roots that were previously grown with 10mM KNO3 was reduced by half. A fraction of the bean root exudates, which is thermolabile and has molecular mass larger than 12kDa stimulated rhizobial adhesion, but this stimulatory activity was lost in root exudates obtained with 10mM KNO3. Thus, the inhibition of symbiosis in response to combined nitrogen may be controlled by the plant at the preinfection stage as well. Springer Science+Business Media Dordrecht, 2013 Nitrate nationallicence Root exudate nationallicence Adhesion nationallicence Rhizobium nationallicence Pérez-Giménez Julieta Instituto de Biotecnología y Biología Molecular. Facultad de Ciencias Exactas, Universidad Nacional de La Plata y CCT-La Plata CONICET, Calles 47 y 115, 1900, La Plata, Argentina aut Lodeiro Aníbal Instituto de Biotecnología y Biología Molecular. Facultad de Ciencias Exactas, Universidad Nacional de La Plata y CCT-La Plata CONICET, Calles 47 y 115, 1900, La Plata, Argentina aut Symbiosis Springer Netherlands 59/3(2013-03-01), 157-163 0334-5114 59:3<157 2013 59 13199 https://doi.org/10.1007/s13199-013-0229-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13199-013-0229-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pérez-Giménez Julieta Instituto de Biotecnología y Biología Molecular. Facultad de Ciencias Exactas, Universidad Nacional de La Plata y CCT-La Plata CONICET, Calles 47 y 115, 1900, La Plata, Argentina aut NATIONALLICENCE 700 1- Lodeiro Aníbal Instituto de Biotecnología y Biología Molecular. Facultad de Ciencias Exactas, Universidad Nacional de La Plata y CCT-La Plata CONICET, Calles 47 y 115, 1900, La Plata, Argentina aut NATIONALLICENCE 773 0- Symbiosis Springer Netherlands 59/3(2013-03-01), 157-163 0334-5114 59:3<157 2013 59 13199 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer