naa a22 4500 510746837 CHVBK 20180411083049.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s13199-012-0213-z doi (NATIONALLICENCE)springer-10.1007/s13199-012-0213-z A new system using Solanum tuberosum for the co-cultivation of Glomus intraradices and its potential for mass producing spores of arbuscular mycorrhizal fungi [Elektronische Daten] [Anshul Puri, Alok Adholeya] Freshly harvested potato tubers, Solanum tuberosum var ‘Pukhraj', were inoculated for transformation with Agrobacterium rhizogenes strain Ri1600. Hairy roots were formed after 8days of co-cultivation and the transformation efficiency was 40%. The transformants were transferred from Murashige and Skoog medium (MS) to Modified White's medium (MW) and finally on a hormone-free minimal medium (M). The putative transformants were confirmed using rolA and rolB gene specific primers for the polymerase chain reaction (PCR) analysis. The root inducing (Ri) T-DNA transformed potato roots were co-cultured with Glomus intraradices (CMCCROC7) to obtain arbuscular mycorrhizal root organ cultures (AM-ROC dual cultures), which were used for studying the symbiosis with Glomus intraradices and the potential for spore production in vitro. Sporulation was comparable with the existing in vitro carrot-dual culture system. Around 60,250 spores/jar could be harvested with around 38,314 extraradical spores/jar and around 21,936 intraradical spores/jar. The new method using potato is certainly promising for the mass production of mycorrhizal biofertilizers. The viability of the spores when tested on potato roots was nearly 100% and more than half of the roots were colonized 12weeks after inoculation. Springer Science+Business Media Dordrecht, 2013 Acetosyringone nationallicence Agrobacterium rhizogenes nationallicence Glomus intraradices nationallicence Inocula nationallicence Root Organ Culture nationallicence Solanum tuberosum nationallicence Sporulation nationallicence Transformation nationallicence AM : Arbuscular mycorrhiza nationallicence AMF : Arbuscular mycorrhizal fungus nationallicence CMCC : Centre for Mycorrhizal Culture Collection nationallicence CTAB : Cetyl trimethyl ammonium bromide nationallicence INT : Indo nitro tetrazolium nationallicence M : Minimal medium nationallicence MS : Murashige and Skoog medium nationallicence MTT : 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H- tetrazolium bromide nationallicence MW : Modified White's medium nationallicence NBT : Nitro blue tetrazolium nationallicence PCR : Polymerase chain reaction nationallicence Ri : Root inducing nationallicence ROC : Root organ culture nationallicence SPSS : Statistical package for the social sciences nationallicence T-DNA : Transfer DNA nationallicence VAM : Vesicular arbuscular mycorrhiza nationallicence YMA : Yeast mannitol agar nationallicence YMB : Yeast mannitol broth nationallicence Puri Anshul Centre for Bioresources and Biotechnology, Teri University, 10, Institutional Area, 110070, Vasant Kunj, New Delhi, India aut Adholeya Alok Centre for Mycorrhizal Research, Biotechnology and Bioresources Division, The Energy and Resources Institute, DS Block, India Habitat Centre, Lodhi Road, 110 003, New Delhi, India aut Symbiosis Springer Netherlands 59/2(2013-02-01), 87-97 0334-5114 59:2<87 2013 59 13199 https://doi.org/10.1007/s13199-012-0213-z text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13199-012-0213-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Puri Anshul Centre for Bioresources and Biotechnology, Teri University, 10, Institutional Area, 110070, Vasant Kunj, New Delhi, India aut NATIONALLICENCE 700 1- Adholeya Alok Centre for Mycorrhizal Research, Biotechnology and Bioresources Division, The Energy and Resources Institute, DS Block, India Habitat Centre, Lodhi Road, 110 003, New Delhi, India aut NATIONALLICENCE 773 0- Symbiosis Springer Netherlands 59/2(2013-02-01), 87-97 0334-5114 59:2<87 2013 59 13199 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer