naa a22 4500 510746888 CHVBK 20180411083049.0 cr unu---uuuuu 180411e20130501xx s 000 0 eng 10.1007/s13199-013-0231-5 doi (NATIONALLICENCE)springer-10.1007/s13199-013-0231-5 Escherichia coli expressing a green fluorescent protein (GFP) in Anopheles stephensi [Elektronische Daten] a preliminary model for paratransgenesis [Ali Chavshin, Mohammad Oshaghi, Hasan Vatandoost, Bagher Yakhchali, Ahmad Raeisi, Fahimeh Zarenejad] Issues, like emerging insecticide resistance in Anopheles mosquitoes, have led to a breakdown in many vector control programs. In this study, a recombinant Escherichia coli with plasmid expressing a green fluorescent protein (E.coli-GFP) was used as a paratransgenesis model to determine: the possibility of E. coli-GFP trans-stadial transmission. The effect of the water microflora, of bacteria-impregnated sugar solutions, and of blood-feeding on E. coli-GFP colonization and localization within An. stephensi tissues, were studied. The results demonstrated the persistence of E. coli-GFP during molting and metamorphosis events and its trans-stadial transmission. Also the efficacy of bacteria-impregnated sugar solutions for transferring the bacteria to the adult mosquito's midgut was shown. A blood meal dramatically increased the number of bacteria within 24-48h post feeding. In addition to fluorescence microscope evaluation, GFP gene PCR amplification showed the presence of the bacteria in the midgut of larvae, pupae, and adults up to 13days after eclosion. Massive colonization of bacteria was observed in the larvae and in the adult mosquito's malpighian tubules which may play a role in retaining bacteria in adult mosquitos. The results of this study showed that E. coli could be used as a laboratory model in paratransgenesis studies for the evaluation of various effector molecules as anti-parasite agents for malaria and filariasis. Springer Science+Business Media Dordrecht, 2013 GFP nationallicence E. coli nationallicence Anopheles stephensi nationallicence Paratransgenesis nationallicence Malaria nationallicence Chavshin Ali Department of Medical Entomology and Vector Control, School of Public Health, Urmia University of Medical Sciences (UMSU), Urmia, Iran aut Oshaghi Mohammad Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences (TUMS), Tehran, Iran aut Vatandoost Hasan Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences (TUMS), Tehran, Iran aut Yakhchali Bagher Department of Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran aut Raeisi Ahmad The malaria office, Center for Diseases Control (CDC), Ministry of Health, Tehran, Iran aut Zarenejad Fahimeh Department of Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran aut Symbiosis Springer Netherlands 60/1(2013-05-01), 17-24 0334-5114 60:1<17 2013 60 13199 https://doi.org/10.1007/s13199-013-0231-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13199-013-0231-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chavshin Ali Department of Medical Entomology and Vector Control, School of Public Health, Urmia University of Medical Sciences (UMSU), Urmia, Iran aut NATIONALLICENCE 700 1- Oshaghi Mohammad Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences (TUMS), Tehran, Iran aut NATIONALLICENCE 700 1- Vatandoost Hasan Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences (TUMS), Tehran, Iran aut NATIONALLICENCE 700 1- Yakhchali Bagher Department of Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran aut NATIONALLICENCE 700 1- Raeisi Ahmad The malaria office, Center for Diseases Control (CDC), Ministry of Health, Tehran, Iran aut NATIONALLICENCE 700 1- Zarenejad Fahimeh Department of Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran aut NATIONALLICENCE 773 0- Symbiosis Springer Netherlands 60/1(2013-05-01), 17-24 0334-5114 60:1<17 2013 60 13199 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer