naa a22 4500 510746918 CHVBK 20180411083049.0 cr unu---uuuuu 180411e20130501xx s 000 0 eng 10.1007/s13199-013-0233-3 doi (NATIONALLICENCE)springer-10.1007/s13199-013-0233-3 Kodama Yuuki Department of Biological Science, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu-cho, 690-8504, Matsue-shi, Shimane, Japan aut Localization of attachment area of the symbiotic Chlorella variabilis of the ciliate Paramecium bursaria during the algal removal and reinfection [Elektronische Daten] [Yuuki Kodama] Chlorella spp. and ciliate Paramecium bursaria share a mutual symbiosis. However, both alga-removed P. bursaria and isolated symbiotic algae can grow independently. Additionally, mixing them experimentally can cause algal reinfection through host phagocytosis. Although the symbiotic algal localization beneath the host cell cortex is a prerequisite phenomenon for maintenance of the relationship of their endosymbiosis, how and where the algae locate beneath the host cell cortex remains unknown. To elucidate this phenomenon, algal distribution patterns during algal removal and reinfection were observed. During algal removal, algae at the host anterior cortex were easier to remove than at the posterior and ventral or dorsal cortex areas. During algal reinfection, the algae after separation from the host digestive vacuoles tended to localize beneath the host ventral or dorsal cortex more readily than that at other cortices. Algae that reinfected trichocyst-removed paramecia didn't show this localization. Trichocyst-discharge experiments clarified that trichocysts of the anterior cortex are difficult to remove. In 14 strains of P. bursaria, some of the paramecia lacked their symbiotic algae at the anterior cortex. These observations demonstrate that symbiotic algae of P. bursaria are difficult to localize at the anterior cortex and that they are easy to remove from the area. Springer Science+Business Media Dordrecht, 2013 Chlorella spp nationallicence Endosymbiosis nationallicence Localization nationallicence Paramecium bursaria nationallicence Trichocysts nationallicence Symbiosis Springer Netherlands 60/1(2013-05-01), 25-36 0334-5114 60:1<25 2013 60 13199 https://doi.org/10.1007/s13199-013-0233-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13199-013-0233-3 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Kodama Yuuki Department of Biological Science, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu-cho, 690-8504, Matsue-shi, Shimane, Japan aut NATIONALLICENCE 773 0- Symbiosis Springer Netherlands 60/1(2013-05-01), 25-36 0334-5114 60:1<25 2013 60 13199 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer