naa a22 4500 510746926 CHVBK 20180411083049.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s13199-013-0248-9 doi (NATIONALLICENCE)springer-10.1007/s13199-013-0248-9 Hevea brasiliensis and Urtica dioica impact the in vitro mycorrhization of neighbouring Medicago truncatula seedlings [Elektronische Daten] [Tiffany Sosa-Rodriguez, Stéphane Declerck, Francoise Granet, Sylvie Gaurel, Els Van Damme, Hervé Dupré de Boulois] Hevea brasiliensis is a mycotrophic tree for which root colonization by arbuscular mycorrhizal fungi (AMF) under in vitro or pot culture conditions can take several weeks. The reason for this slow colonization is still unknown, but the exudation of antifungal compounds such as hevein by the roots may be one of the causes. Here, the root colonization of Medicago truncatula, a highly mycotrophic plant, was assessed after 12days of growth in the extraradical mycelium network of the AMF Rhizophagus irregularis in close vicinity of H. brasiliensis plantlets or Urtica dioica seedlings (also known to synthesize antifungal compounds of the hevein family). We hypothesized that a negative impact on the root colonization of a M. truncatula seedling developing close to H. brasiliensis and U. dioica may give indirect proofs for the exudation of inhibitory molecules. The percentages of total root colonization of M. truncatula were 30.1% lower in the presence of H. brasiliensis than in the control plants, and 29.1% lower in presence of U. dioica. The abundance of arbuscules in the roots of M. truncatula was also lower in plants grown in presence of H. brasiliensis plantlets than in the control plants. Similarly, the succinate dehydrogenase and the phosphatase activities measured in the extraradical mycelium of R. irregularis were significantly lower in the presence of both plants, compared with the controls. No root colonization was observed in H. brasiliensis and U. dioica within the time-frame of the experiments. The low root colonization of M. truncatula when grown in the presence of rubber or stinging nettle suggested the exudation of diffusible molecules which could also explain the delayed root colonization of H. brasiliensis and the absence of colonization of U. dioica. Springer Science+Business Media Dordrecht, 2013 Arbuscular mycorrhizal fungi nationallicence Hevea brasiliensis nationallicence Urtica dioica nationallicence Medicago truncatula nationallicence Rhizophagus irregularis nationallicence Extraradical mycelium network nationallicence Sosa-Rodriguez Tiffany Université Catholique de Louvain, Earth and Life Institute, Applied Microbiology, Mycology, Croix du Sud 2 bte L7.05.06, 1348, Louvain-la-Neuve, Belgium aut Declerck Stéphane Université Catholique de Louvain, Earth and Life Institute, Applied Microbiology, Mycology, Croix du Sud 2 bte L7.05.06, 1348, Louvain-la-Neuve, Belgium aut Granet Francoise Manufacture Française des Pneumatiques MICHELIN Place des Carmes-Déchaux, 63040, Clermont-Ferrand CEDEX, France aut Gaurel Sylvie Manufacture Française des Pneumatiques MICHELIN Place des Carmes-Déchaux, 63040, Clermont-Ferrand CEDEX, France aut Van Damme Els Department of Molecular Biotechnology, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut Dupré de Boulois Hervé Université Catholique de Louvain, Earth and Life Institute, Applied Microbiology, Mycology, Croix du Sud 2 bte L7.05.06, 1348, Louvain-la-Neuve, Belgium aut Symbiosis Springer Netherlands 60/3(2013-07-01), 123-132 0334-5114 60:3<123 2013 60 13199 https://doi.org/10.1007/s13199-013-0248-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13199-013-0248-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sosa-Rodriguez Tiffany Université Catholique de Louvain, Earth and Life Institute, Applied Microbiology, Mycology, Croix du Sud 2 bte L7.05.06, 1348, Louvain-la-Neuve, Belgium aut NATIONALLICENCE 700 1- Declerck Stéphane Université Catholique de Louvain, Earth and Life Institute, Applied Microbiology, Mycology, Croix du Sud 2 bte L7.05.06, 1348, Louvain-la-Neuve, Belgium aut NATIONALLICENCE 700 1- Granet Francoise Manufacture Française des Pneumatiques MICHELIN Place des Carmes-Déchaux, 63040, Clermont-Ferrand CEDEX, France aut NATIONALLICENCE 700 1- Gaurel Sylvie Manufacture Française des Pneumatiques MICHELIN Place des Carmes-Déchaux, 63040, Clermont-Ferrand CEDEX, France aut NATIONALLICENCE 700 1- Van Damme Els Department of Molecular Biotechnology, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Dupré de Boulois Hervé Université Catholique de Louvain, Earth and Life Institute, Applied Microbiology, Mycology, Croix du Sud 2 bte L7.05.06, 1348, Louvain-la-Neuve, Belgium aut NATIONALLICENCE 773 0- Symbiosis Springer Netherlands 60/3(2013-07-01), 123-132 0334-5114 60:3<123 2013 60 13199 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer