naa a22 4500 510746942 CHVBK 20180411083049.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s13199-013-0247-x doi (NATIONALLICENCE)springer-10.1007/s13199-013-0247-x Genotypic variation of nodules' enzymatic activities in symbiotic nitrogen fixation among common bean ( Phaseolus vulgaris L.) genotypes grown under salinity constraint [Elektronische Daten] [Mustapha Faghire, Farissi Mohamed, Khadijattou Taoufiq, Rachid Fghire, Adnane Bargaz, Btissam Mandri, Khalid Oufdou, Amenc Laury, Jean-Jacques Drevon, Cherki Ghoulam] The effect of salt stress, under glasshouse conditions, was studied on plant biomass, nodulation, and activities of acid phosphatases (APase, EC 3.1.3.2) and trehalose 6-phosphate phosphatase (TPP, EC 3.1.3.12) in the symbiosis common bean (Phaseolus vulgaris L.)-rhizobia nodules. Four common bean recombinant inbred lines (147, 115, 104 and 83) were separately inoculated, with CIAT 899 or RhM11 strains and grown in hydroaeroponic culture. Two NaCl levels (0 and 25mM NaCl plant−1week−1 corresponding, respectively, to the control and the salt treatment) were applied and the culture was assessed during 42days after their transplantation. The results showed that the nodulation of these lines was not affected by salinity except for the line 83 inoculated with CIAT 899, whose nodule dry weight decreased by 48.24% compared with the corresponding controls. For the other symbiotic combinations, shoot and root biomasses were not significantly affected by salt constraint. Salinity stress generally reduced acid phosphatise and trehalose phosphate phosphatase activities in nodules that were less affected in plants inoculated with RhM11. Based on our data, it appears that nodule phosphatase activity may be involved in salinity tolerance in common beans and the levels of salt tolerance depend principally on specific combination of the rhizobial strain and the host cultivar. Springer Science+Business Media Dordrecht, 2013 Common bean nationallicence Nodulation nationallicence Salinity constraint nationallicence Acid phosphatase nationallicence Trehalose phosphate phosphatase nationallicence RT-PCR in situ nationallicence Faghire Mustapha Department of Biology, Faculty of Sciences, Ibn Zohr University, B.P 8106, Agadir, Morocco aut Mohamed Farissi Faculté des Sciences et Techniques Guéliz-Marrakech, Equipe de Biotechnologie Végétale et Agrophysiologie des Symbioses, BP 549, 40000, Marrakech, Morocco aut Taoufiq Khadijattou Centre des Études et Recherches sur l'Espace Marocain (CEREM), Province Tahanaoute, Morocco aut Fghire Rachid Centre des Études et Recherches sur l'Espace Marocain (CEREM), Province Tahanaoute, Morocco aut Bargaz Adnane Department of Biosystems and Technology, Swedish University of Agricultural Sciences, Box 103, 230 53, Alnarp, Sweden aut Mandri Btissam Faculté des Sciences et Techniques Guéliz-Marrakech, Equipe de Biotechnologie Végétale et Agrophysiologie des Symbioses, BP 549, 40000, Marrakech, Morocco aut Oufdou Khalid Faculté des Sciences-Semlalia, Laboratoire de Biologie et de Biotechnologie des Microorganismes, B.P.2390, Marrakech, Morocco aut Laury Amenc INRA-Montpellier-SupAgro, UMR1222, Rhizosphère et Symbioses, 2 Place Viala, 34060, Montpellier, France aut Drevon Jean-Jacques INRA-Montpellier-SupAgro, UMR1222, Rhizosphère et Symbioses, 2 Place Viala, 34060, Montpellier, France aut Ghoulam Cherki Faculté des Sciences et Techniques Guéliz-Marrakech, Equipe de Biotechnologie Végétale et Agrophysiologie des Symbioses, BP 549, 40000, Marrakech, Morocco aut Symbiosis Springer Netherlands 60/3(2013-07-01), 115-122 0334-5114 60:3<115 2013 60 13199 https://doi.org/10.1007/s13199-013-0247-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13199-013-0247-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Faghire Mustapha Department of Biology, Faculty of Sciences, Ibn Zohr University, B.P 8106, Agadir, Morocco aut NATIONALLICENCE 700 1- Mohamed Farissi Faculté des Sciences et Techniques Guéliz-Marrakech, Equipe de Biotechnologie Végétale et Agrophysiologie des Symbioses, BP 549, 40000, Marrakech, Morocco aut NATIONALLICENCE 700 1- Taoufiq Khadijattou Centre des Études et Recherches sur l'Espace Marocain (CEREM), Province Tahanaoute, Morocco aut NATIONALLICENCE 700 1- Fghire Rachid Centre des Études et Recherches sur l'Espace Marocain (CEREM), Province Tahanaoute, Morocco aut NATIONALLICENCE 700 1- Bargaz Adnane Department of Biosystems and Technology, Swedish University of Agricultural Sciences, Box 103, 230 53, Alnarp, Sweden aut NATIONALLICENCE 700 1- Mandri Btissam Faculté des Sciences et Techniques Guéliz-Marrakech, Equipe de Biotechnologie Végétale et Agrophysiologie des Symbioses, BP 549, 40000, Marrakech, Morocco aut NATIONALLICENCE 700 1- Oufdou Khalid Faculté des Sciences-Semlalia, Laboratoire de Biologie et de Biotechnologie des Microorganismes, B.P.2390, Marrakech, Morocco aut NATIONALLICENCE 700 1- Laury Amenc INRA-Montpellier-SupAgro, UMR1222, Rhizosphère et Symbioses, 2 Place Viala, 34060, Montpellier, France aut NATIONALLICENCE 700 1- Drevon Jean-Jacques INRA-Montpellier-SupAgro, UMR1222, Rhizosphère et Symbioses, 2 Place Viala, 34060, Montpellier, France aut NATIONALLICENCE 700 1- Ghoulam Cherki Faculté des Sciences et Techniques Guéliz-Marrakech, Equipe de Biotechnologie Végétale et Agrophysiologie des Symbioses, BP 549, 40000, Marrakech, Morocco aut NATIONALLICENCE 773 0- Symbiosis Springer Netherlands 60/3(2013-07-01), 115-122 0334-5114 60:3<115 2013 60 13199 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer