naa a22 4500 510746969 CHVBK 20180411083049.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s13199-013-0251-1 doi (NATIONALLICENCE)springer-10.1007/s13199-013-0251-1 Tomato ethylene mutants exhibit differences in arbuscular mycorrhiza development and levels of plant defense-related transcripts [Elektronische Daten] [Giselle Fracetto, Lázaro Peres, Mona Mehdy, Marcio Lambais] The role of ethylene on arbuscular mycorrhiza (AM) development remains unclear. We used tomato ethylene mutants in near isogenic genotypes to evaluate AM development and growth differences and whether changes in levels of plant defense-related transcripts in roots were correlated with those differences. The cultivar Micro-Tom (MT) and mutants overproducing ethylene (epinastic, epi) and insensitive to ethylene (Never-ripe, Nr) were inoculated with Glomus clarum. The epi mutants exhibited reduced intraradical colonization at all time-points and reduced frequencies of hyphopodia and arbuscules at 30 and 37days after inoculation (DAI) compared to MT. At these times and 23 DAI, epi roots contained higher levels of mRNAs encoding Chi3 and Chi9 chitinases, Cu-Zn superoxide dismutase, catalase and a peroxidase than MT roots. In contrast, Nr showed higher intraradical colonization and frequencies of arbuscules, external hypha and hyphopodia but only at 23 DAI. At the preceding 16 DAI time point, levels of antioxidant enzyme mRNAs were higher while Chi3, Chi9 and GlucB mRNAs were lower than in MT roots. Our study provides evidence that specific stages of AM development were altered negatively in epi and positively in Nr. These effects on fungal growth are most likely due to altered ethylene signaling modulating the plant defense system. Springer Science+Business Media Dordrecht, 2013 Arbuscular mycorrhiza nationallicence Micro-Tom nationallicence Ethylene nationallicence Plant defense genes nationallicence Oxidative stress nationallicence Fracetto Giselle Departamento de Ciência do Solo, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Av. Pádua Dias, 11, 13418-900, Piracicaba, São Paulo, Brazil aut Peres Lázaro Laboratório de Controle Hormonal do Desenvolvimento Vegetal, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Av. Pádua Dias, 11, 13418-900, Piracicaba, São Paulo, Brazil aut Mehdy Mona Section of Molecular, Cellular and Developmental Biology and Institute for Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station A6700, 78712, Austin, TX, USA aut Lambais Marcio Departamento de Ciência do Solo, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Av. Pádua Dias, 11, 13418-900, Piracicaba, São Paulo, Brazil aut Symbiosis Springer Netherlands 60/3(2013-07-01), 155-167 0334-5114 60:3<155 2013 60 13199 https://doi.org/10.1007/s13199-013-0251-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13199-013-0251-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fracetto Giselle Departamento de Ciência do Solo, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Av. Pádua Dias, 11, 13418-900, Piracicaba, São Paulo, Brazil aut NATIONALLICENCE 700 1- Peres Lázaro Laboratório de Controle Hormonal do Desenvolvimento Vegetal, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Av. Pádua Dias, 11, 13418-900, Piracicaba, São Paulo, Brazil aut NATIONALLICENCE 700 1- Mehdy Mona Section of Molecular, Cellular and Developmental Biology and Institute for Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station A6700, 78712, Austin, TX, USA aut NATIONALLICENCE 700 1- Lambais Marcio Departamento de Ciência do Solo, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Av. Pádua Dias, 11, 13418-900, Piracicaba, São Paulo, Brazil aut NATIONALLICENCE 773 0- Symbiosis Springer Netherlands 60/3(2013-07-01), 155-167 0334-5114 60:3<155 2013 60 13199 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer