naa a22 4500 510747000 CHVBK 20180411083050.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s13199-013-0242-2 doi (NATIONALLICENCE)springer-10.1007/s13199-013-0242-2 Natural variation in population densities and vertical transmission rates of a Spiroplasma endosymbiont in Drosophila hydei [Elektronische Daten] [Ryu Osaka, Temma Ichizono, Daisuke Kageyama, Masashi Nomura, Masayoshi Watada] A bacterium belonging to the genus Spiroplasma, an endosymbiont of the fly Drosophila hydei, is vertically transmitted through host egg cytoplasm. To infer vertical transmission rates of Spiroplasma in natural populations of D. hydei, the infection status of Spiroplasma was examined for offspring produced by Spiroplasma-positive females that were collected in two geographical populations. In both populations, nearly half of the broods consisted of only infected offspring. Infection frequencies of the rest of the broods ranged from 0.364 to 0.975. Quantitative PCR demonstrated that the Spiroplasma titers in the whole body of wild-caught females were highly variable (1.81 × 106-5.60 × 108 cells per insect). Contrary to our expectations, however, the Spiroplasma titers did not account for the variation in infection frequencies among offspring (i.e., vertical transmission rates). These results suggest that the spatial distribution of Spiroplasma, particularly in somatic tissues and germ tissues, is highly variable among host individuals, which may be caused by environmental stochasticity or some unknown effects. Springer Science+Business Media Dordrecht, 2013 Drosophila hydei nationallicence Endosymbiont nationallicence Infection density nationallicence Spiroplasma nationallicence Vertical transmission nationallicence Osaka Ryu Graduate School of Horticulture, Chiba University, Matsudo, Chiba, Japan aut Ichizono Temma Graduate School of Science and Engineering, Ehime University, Matsuyama, Ehime, Japan aut Kageyama Daisuke National Institute of Agrobiological Sciences (NIAS), 1-2, Owashi, 305-8634, Tsukuba, Ibaraki, Japan aut Nomura Masashi Graduate School of Horticulture, Chiba University, Matsudo, Chiba, Japan aut Watada Masayoshi Graduate School of Science and Engineering, Ehime University, Matsuyama, Ehime, Japan aut Symbiosis Springer Netherlands 60/2(2013-06-01), 73-78 0334-5114 60:2<73 2013 60 13199 https://doi.org/10.1007/s13199-013-0242-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13199-013-0242-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Osaka Ryu Graduate School of Horticulture, Chiba University, Matsudo, Chiba, Japan aut NATIONALLICENCE 700 1- Ichizono Temma Graduate School of Science and Engineering, Ehime University, Matsuyama, Ehime, Japan aut NATIONALLICENCE 700 1- Kageyama Daisuke National Institute of Agrobiological Sciences (NIAS), 1-2, Owashi, 305-8634, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Nomura Masashi Graduate School of Horticulture, Chiba University, Matsudo, Chiba, Japan aut NATIONALLICENCE 700 1- Watada Masayoshi Graduate School of Science and Engineering, Ehime University, Matsuyama, Ehime, Japan aut NATIONALLICENCE 773 0- Symbiosis Springer Netherlands 60/2(2013-06-01), 73-78 0334-5114 60:2<73 2013 60 13199 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer