naa a22 4500 510747027 CHVBK 20180411083050.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s13199-013-0241-3 doi (NATIONALLICENCE)springer-10.1007/s13199-013-0241-3 Detection of Spiroplasma from the mite Macrocheles sp. (Acari; Macrochelidae) ectoparasitic to the fly Drosophila hydei (Diptera; Drosophilidae): a possible route of horizontal transmission? [Elektronische Daten] [Ryu Osaka, Masayoshi Watada, Daisuke Kageyama, Masashi Nomura] Some members of the genus Spiroplasma are vertically transmitted endosymbionts of insects. Among them, Spiroplasma sp. Dhd, a member of the Spiroplasma poulsonii clade, is highly prevalent among worldwide populations of Drosophila hydei. Here we found that 53 out of 3,763 wild-caught D. hydei (1.4%) were ectoparasitized by the mite that belong to the genus Macrocheles. Many of the ectoparasitized flies (79%) had a single mite, but some flies had up to five mites. Among 59 mites subjected to Spiroplasma-specific PCR, 15 individuals were found to be positive. Infection status of Spiroplasma in flies and the associated mites were incongruent. Partial nucleotide sequences of the Spiroplasma P58 gene suggest that some of the mites are infected with a Spiroplasma, which is identical or closely related to Spiroplasma sp. Dhd. This finding provides a potential route of horizontal Spiroplasma transmission between D. hydei individuals in natural populations. In addition, a Spiroplasma strain that does not form a monophyletic group with S. poulsonii was also found from a mite individual. Springer Science+Business Media Dordrecht, 2013 Drosophila hydei nationallicence Ectoparasite nationallicence Endosymbiont nationallicence Macrocheles nationallicence Spiroplasma nationallicence Osaka Ryu Graduate School of Horticulture, Chiba University, Matsudo, Chiba, Japan aut Watada Masayoshi Graduate School of Science and Engineering, Ehime University, Matsuyama, Ehime, Japan aut Kageyama Daisuke National Institute of Agrobiological Sciences (NIAS), Tsukuba, Ibaraki, Japan aut Nomura Masashi Graduate School of Horticulture, Chiba University, Matsudo, Chiba, Japan aut Symbiosis Springer Netherlands 60/2(2013-06-01), 79-84 0334-5114 60:2<79 2013 60 13199 https://doi.org/10.1007/s13199-013-0241-3 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13199-013-0241-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Osaka Ryu Graduate School of Horticulture, Chiba University, Matsudo, Chiba, Japan aut NATIONALLICENCE 700 1- Watada Masayoshi Graduate School of Science and Engineering, Ehime University, Matsuyama, Ehime, Japan aut NATIONALLICENCE 700 1- Kageyama Daisuke National Institute of Agrobiological Sciences (NIAS), Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Nomura Masashi Graduate School of Horticulture, Chiba University, Matsudo, Chiba, Japan aut NATIONALLICENCE 773 0- Symbiosis Springer Netherlands 60/2(2013-06-01), 79-84 0334-5114 60:2<79 2013 60 13199 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer