naa a22 4500 510747035 CHVBK 20180411083050.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s13199-013-0244-0 doi (NATIONALLICENCE)springer-10.1007/s13199-013-0244-0 Symbiotic influence of endophytic Bacillus pumilus on growth promotion and probiotic potential of the medicinal plant Ocimum sanctum [Elektronische Daten] [RM. Murugappan, S. Begum, R. Roobia] Bacillus pumilus was isolated from surface-sterilized tissues of the medicinal plant Ocimum sanctum. Scanning electron microscopic (SEM) imaging confirmed the presence of a rod shaped bacterium within the plant tissues. The bacterium was identified as B. pumilus by biochemical analyses and 16S rRNA gene sequencing. In vitro analyses indicate that the isolated strain of B. pumilus was endowed with multiple plant growth promotion (PGP) traits such as phosphate solubilization and the production of indole acetic acid (IAA), siderophore and hydrogen cyanide (HCN). Phosphate solubilization (37.3μgml−1) and IAA production (36.7μgml−1) by the isolate was found to reach a maximum after 60h of incubation. Siderophore mediated iron sequestration by B. pumilus may confer a competitive advantage to the host with respect to pathogen inhibition. Siderophore produced by the isolate was found to be of a trihydroxamate type with hexadentate nature. The B. pumilus isolate also exhibited cellulolytic, proteolytic and chitinolytic activity. Cell free supernatant, culture filtrates of the isolate were found to suppress the growth of fungal phytopathogens. The culture filtrate retained its antifungal activity even after exposure to heat. In addition to PGP, the isolate exhibited probiotic properties such as acid tolerance (pH2), bile salt tolerance (2%), auto-aggregation, antibiotic resistance and the absence of haemolytic activity. These finding suggest the possibility of utilizing this endophytic strain of B. pumilus as a bioinoculant to enhance plant growth and also as a probiotic. Springer Science+Business Media Dordrecht, 2013 Ocimum sanctum nationallicence Bacillus pumilus nationallicence Plant growth promotion nationallicence Siderophore nationallicence Probiotics nationallicence Murugappan RM Department of Zoology and Microbiology, Thiagarajar College, Madurai, 625 009, Tamil Nadu, India aut Begum S. Department of Zoology and Microbiology, Thiagarajar College, Madurai, 625 009, Tamil Nadu, India aut Roobia R. Department of Zoology and Microbiology, Thiagarajar College, Madurai, 625 009, Tamil Nadu, India aut Symbiosis Springer Netherlands 60/2(2013-06-01), 91-99 0334-5114 60:2<91 2013 60 13199 https://doi.org/10.1007/s13199-013-0244-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13199-013-0244-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Murugappan RM Department of Zoology and Microbiology, Thiagarajar College, Madurai, 625 009, Tamil Nadu, India aut NATIONALLICENCE 700 1- Begum S. Department of Zoology and Microbiology, Thiagarajar College, Madurai, 625 009, Tamil Nadu, India aut NATIONALLICENCE 700 1- Roobia R. Department of Zoology and Microbiology, Thiagarajar College, Madurai, 625 009, Tamil Nadu, India aut NATIONALLICENCE 773 0- Symbiosis Springer Netherlands 60/2(2013-06-01), 91-99 0334-5114 60:2<91 2013 60 13199 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer