naa a22 4500 51074883X CHVBK 20180411083057.0 cr unu---uuuuu 180411e20130101xx s 000 0 eng 10.1007/s11270-012-1382-3 doi (NATIONALLICENCE)springer-10.1007/s11270-012-1382-3 High Polyacrylamide Application Rates Do Not Affect Eubacterial Structural Diversity [Elektronische Daten] [James Entry, DeEtta Mills, Krish Jayachandran, R. Sojka] Anionic polyacrylamide (PAM) is a linear, water-soluble anionic polymer that is widely used for erosion control and water quality protection. There has been an issue whether this formulation could possibly have negative effects on soil microbial diversity by altering microbial binding to soil particles or to one another and thus restricting their mobility and role in carbon and nutrient cycling. We conducted an 8-year study annually applying ultra-high rates of PAM to soil and then monitored impacts on soil bacterial diversity. In July and August, we measured active soil bacterial and fungal biomass and microbial diversity in soils receiving 0 (control), 2,691, and 5,382kg active ingredient PAM ha−1. Active microbial biomass in soil was 19-33% greater in the untreated control than soil treated with 2,691 or 5,382kg of active ingredient PAM ha−1. Active bacterial biomass in soil was 21-31% greater in the control treatment than in soil treated with 2,691 or 5,382kg of active ingredient PAM ha−1 in August, but not July. Active fungal biomass in soils was 38-50% greater in the control treatment than soil treated with 2,691 or 5,382kg of active ingredient PAM ha−1 in July, but not August. Molecular methods were used to access the bacterial diversity, richness, and evenness in an agricultural soil that received 0 (control), 2,691, and 5,382kg of active ingredient PAM ha−1. We found that although soil receiving these massive PAM application rates and prolonged exposure may reduce active bacterial and fungal biomass, PAM application did not substantially or consistently affect bacterial structural diversity, richness, or evenness in this agricultural soil. Springer Science+Business Media Dordrecht, 2012 Anionic polyacrylamide nationallicence DNA nationallicence Amplicon length heterogeneity nationallicence Bacterial diversity nationallicence Entry James USDA Agricultural Research Service, Northwest Irrigation and Soils Research Laboratory, 3793 North 3600 East, 83341, Kimberly, ID, USA aut Mills DeEtta Department of Biological Sciences, Florida International University, University Park, Miami, FL, USA aut Jayachandran Krish Department of Earth and Environmental Sciences, Florida International University, University Park, Miami, FL, USA aut Sojka R. USDA Agricultural Research Service, Northwest Irrigation and Soils Research Laboratory, 3793 North 3600 East, 83341, Kimberly, ID, USA aut Water, Air, & Soil Pollution Springer Netherlands 224/1(2013-01-01), 1-10 0049-6979 224:1<1 2013 224 11270 https://doi.org/10.1007/s11270-012-1382-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11270-012-1382-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Entry James USDA Agricultural Research Service, Northwest Irrigation and Soils Research Laboratory, 3793 North 3600 East, 83341, Kimberly, ID, USA aut NATIONALLICENCE 700 1- Mills DeEtta Department of Biological Sciences, Florida International University, University Park, Miami, FL, USA aut NATIONALLICENCE 700 1- Jayachandran Krish Department of Earth and Environmental Sciences, Florida International University, University Park, Miami, FL, USA aut NATIONALLICENCE 700 1- Sojka R. USDA Agricultural Research Service, Northwest Irrigation and Soils Research Laboratory, 3793 North 3600 East, 83341, Kimberly, ID, USA aut NATIONALLICENCE 773 0- Water, Air, & Soil Pollution Springer Netherlands 224/1(2013-01-01), 1-10 0049-6979 224:1<1 2013 224 11270 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer