naa a22 4500 51074916X CHVBK 20180411083058.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s11270-013-1587-0 doi (NATIONALLICENCE)springer-10.1007/s11270-013-1587-0 A Novel Bacterium That Degrades Aroclor-1254 and Its bphC Gene Encodes an Extradiol Aromatic Ring Cleavage Dioxygenase (EARCD) [Elektronische Daten] [Selvaraj Ganesh-Kumar, Karuppanan Kalimuthu, Solomon Jebakumar] The congener-specific degradation pattern of polychlorinated biphenyls (PCBs) in Aroclor 1254 was investigated using a novel bacterial strain, Stenotrophomonas sp. JSG1, and it was accelerated by the surfactant, β-cyclodextrin. In addition, 4-chorobenzoic acid (CBA) degradation was also confirmed by the estimation of CBA depletion rate, microbial growth, and release of free chloride ion in mineral medium. Metal ions such as Ni2+, Hg2+ Ba2+, Cu2+, and NaCl (>4%) were found greatly influencing the PCB degradation. However, Ca2+, Mg2+, Fe2+, and Mn2+ have not shown any impact on biodegradation. The bphC gene, which encodes an extradiol aromatic ring cleavage dioxygenase was successfully amplified and cloned. Phylogeny-based pairwise alignment of nucleotide sequences suggested that the cloned gene belongs to the extradiol dioxygenase family, but it showed high diversity to the traditional bphC gene. Results of the present investigation revealed that the Stenotrophomonas sp. JSG1 is an effective novel bacterium, which can be used in the PCB remediation studies. Springer Science+Business Media Dordrecht, 2013 Polychlorinated biphenyl nationallicence Chlorobenzoic acid nationallicence Chloride ion nationallicence Metal ions nationallicence Extradiol dioxygenase nationallicence β-Cyclodextrin nationallicence Ganesh-Kumar Selvaraj Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai-21, Tamil Nadu, India aut Kalimuthu Karuppanan Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai-21, Tamil Nadu, India aut Jebakumar Solomon Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai-21, Tamil Nadu, India aut Water, Air, & Soil Pollution Springer Netherlands 224/6(2013-06-01), 1-12 0049-6979 224:6<1 2013 224 11270 https://doi.org/10.1007/s11270-013-1587-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11270-013-1587-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ganesh-Kumar Selvaraj Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai-21, Tamil Nadu, India aut NATIONALLICENCE 700 1- Kalimuthu Karuppanan Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai-21, Tamil Nadu, India aut NATIONALLICENCE 700 1- Jebakumar Solomon Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai-21, Tamil Nadu, India aut NATIONALLICENCE 773 0- Water, Air, & Soil Pollution Springer Netherlands 224/6(2013-06-01), 1-12 0049-6979 224:6<1 2013 224 11270 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer