naa a22 4500 510749194 CHVBK 20180411083058.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s11270-013-1595-0 doi (NATIONALLICENCE)springer-10.1007/s11270-013-1595-0 Microbial Decolorization of an Azo Dye Reactive Black 5 Using White-Rot Fungus Pleurotus eryngii F032 [Elektronische Daten] [Tony Hadibarata, Liyana Adnan, Abdull Yusoff, Adhi Yuniarto, Rubiyatno,, Meor Zubir, Ameer Khudhair, Zee Teh, M. Naser] The growth of white-rot fungus Pleurotus eryngii F032 in a suitable medium can degrade an azo dye Reactive Black 5 (RB5), because of its ability to produce ligninolytic enzymes such as lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase that able to degrade and transform the complex structure of the dye into a less toxic compound. The effect of environmental factors such as initial concentration of Reactive Black 5, pH, temperature of growth medium, surfactant (Tween 80), and agitation were also investigated. The productions of ligninolytic enzymes were enhanced by increasing the white-rot fungi growth in optimum conditions. The decolorization of Reactive Black 5 were analyzed by using UV-vis spectrophotometer at the maximum absorbance of 596nm. The white-rot fungus, P. eryngii F032 culture exhibited 93.56% decolorization of 10mg/L RB5 within 72h of incubation in dark condition with agitation. The optimum pH and temperature for the decolorizing activity was recorded at pH3 and 40°C, respectively. The addition of surfactant (Tween 80) increased the decolorization to 93.57% and agitation of growth medium at 120rpm enhanced the distribution of nutrients to the fungus thus optimized the enzymatic reaction that resulted maximum decolorization of RB5 which was 93.57%. The molecular docking studies were performed using Chimera visualization software as to analyze the decolorization mechanism of RB5 at molecular level. Springer Science+Business Media Dordrecht, 2013 Pleurotus eryngii F032 nationallicence Reactive Black 5 nationallicence Decolorization nationallicence Tween 80 nationallicence Environmental factors nationallicence Chimera visualization software nationallicence Hadibarata Tony Institute of Environmental and Water Resources Management, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Malaysia aut Adnan Liyana Institute of Environmental and Water Resources Management, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Malaysia aut Yusoff Abdull Institute of Environmental and Water Resources Management, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Malaysia aut Yuniarto Adhi Institute of Environmental and Water Resources Management, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Malaysia aut Rubiyatno, Institute of Environmental and Water Resources Management, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Malaysia aut Zubir Meor Institute of Environmental and Water Resources Management, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Malaysia aut Khudhair Ameer Institute of Environmental and Water Resources Management, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Malaysia aut Teh Zee Institute of Environmental and Water Resources Management, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Malaysia aut Naser M. Department of Biosciences and Health Sciences, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Malaysia aut Water, Air, & Soil Pollution Springer Netherlands 224/6(2013-06-01), 1-9 0049-6979 224:6<1 2013 224 11270 https://doi.org/10.1007/s11270-013-1595-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11270-013-1595-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hadibarata Tony Institute of Environmental and Water Resources Management, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Malaysia aut NATIONALLICENCE 700 1- Adnan Liyana Institute of Environmental and Water Resources Management, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Malaysia aut NATIONALLICENCE 700 1- Yusoff Abdull Institute of Environmental and Water Resources Management, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Malaysia aut NATIONALLICENCE 700 1- Yuniarto Adhi Institute of Environmental and Water Resources Management, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Malaysia aut NATIONALLICENCE 700 1- Rubiyatno Institute of Environmental and Water Resources Management, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Malaysia aut NATIONALLICENCE 700 1- Zubir Meor Institute of Environmental and Water Resources Management, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Malaysia aut NATIONALLICENCE 700 1- Khudhair Ameer Institute of Environmental and Water Resources Management, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Malaysia aut NATIONALLICENCE 700 1- Teh Zee Institute of Environmental and Water Resources Management, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Malaysia aut NATIONALLICENCE 700 1- Naser M. Department of Biosciences and Health Sciences, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Malaysia aut NATIONALLICENCE 773 0- Water, Air, & Soil Pollution Springer Netherlands 224/6(2013-06-01), 1-9 0049-6979 224:6<1 2013 224 11270 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer