naa a22 4500 510749704 CHVBK 20180411083100.0 cr unu---uuuuu 180411e20130901xx s 000 0 eng 10.1007/s11270-013-1648-4 doi (NATIONALLICENCE)springer-10.1007/s11270-013-1648-4 Isolation and Identification of Toluene-Metabolizing Bacteria from Rhizospheres of Two Indoor Plants [Elektronische Daten] [Hao Zhang, Svoboda Pennisi, Stanley Kays, Mussie Habteselassie] The role of the rhizosphere microbial community in removing volatile organic compounds has not been well investigated. In this study, two species of indoor foliage plants, Fittonia verschaffeltii var. argyroneura and Hoya carnosa, were primed with toluene exposure for 2months, followed by isolation and identification of the rhizosphere bacteria that were demonstrated to metabolize toluene. A total of 42 bacterial isolates were obtained. The number of bacterial isolates was narrowed down to 23, which had banding pattern similarities of 80% or less, using BOX-polymerase chain reaction (PCR) fingerprinting technique. The 23 isolates were further characterized by sequencing part of their 16S rDNA after PCR. Their identities were examined using Basic Local Alignment Search Tool (BLAST), resulting in the isolates having the highest sequence similarities (97-100%) to eight known bacteria strains, none of which had been previously reported to be capable of degrading toluene. The bacterial isolates were positive for toluene monooxygenase gene, confirming their genetic potential to metabolize toluene. Five of the isolates were further tested with 14C-labeled toluene to directly show their ability to metabolize toluene. Isolate type did not significantly affect the percent of toluene mineralized over 2weeks' time. However, the isolates had differing response to varying toluene concentrations. Under low (0.05) and high (0.2μCi/mL) concentrations, they mineralized 43 and 49% of toluene, respectively. The isolation and characterization of toluene-metabolizing bacteria corroborates previous speculation that the rhizosphere microbial community contributes to the phytoremediation potential of indoor foliage plants. Springer Science+Business Media Dordrecht, 2013 Rhizosphere bacteria nationallicence Phytoremediation nationallicence Indoor air quality nationallicence Foliage plants nationallicence Zhang Hao Department of Crop and Soil Sciences, The University of Georgia—Griffin Campus, 1109 Experiment St, 30223, Griffin, GA, USA aut Pennisi Svoboda Department of Horticulture, The University of Georgia—Griffin Campus, 1109 Experiment St, 30223, Griffin, GA, USA aut Kays Stanley Department of Horticulture, The University of Georgia, Plant Sciences Building, Athens, GA, USA aut Habteselassie Mussie Department of Crop and Soil Sciences, The University of Georgia—Griffin Campus, 1109 Experiment St, 30223, Griffin, GA, USA aut Water, Air, & Soil Pollution Springer Netherlands 224/9(2013-09-01), 1-14 0049-6979 224:9<1 2013 224 11270 https://doi.org/10.1007/s11270-013-1648-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11270-013-1648-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Hao Department of Crop and Soil Sciences, The University of Georgia—Griffin Campus, 1109 Experiment St, 30223, Griffin, GA, USA aut NATIONALLICENCE 700 1- Pennisi Svoboda Department of Horticulture, The University of Georgia—Griffin Campus, 1109 Experiment St, 30223, Griffin, GA, USA aut NATIONALLICENCE 700 1- Kays Stanley Department of Horticulture, The University of Georgia, Plant Sciences Building, Athens, GA, USA aut NATIONALLICENCE 700 1- Habteselassie Mussie Department of Crop and Soil Sciences, The University of Georgia—Griffin Campus, 1109 Experiment St, 30223, Griffin, GA, USA aut NATIONALLICENCE 773 0- Water, Air, & Soil Pollution Springer Netherlands 224/9(2013-09-01), 1-14 0049-6979 224:9<1 2013 224 11270 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer