naa a22 4500 510750109 CHVBK 20180411083101.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s11270-013-1627-9 doi (NATIONALLICENCE)springer-10.1007/s11270-013-1627-9 Acute Copper Toxicity and Acclimation to Copper Using the Behavioral Endpoint of Shoaling, in the Least Killifish ( Heterandria formosa ) [Elektronische Daten] [Sarah Vogt, Arlene Billock, Paul Klerks] While copper is known to be neurotoxic, effects on behavior and especially on group behavior have received much less attention. Yet such behavioral effects can have important ecological consequences. This study determined whether shoaling behavior could be used as an indicator of acute copper toxicity and as an endpoint in studying acclimation in the least killifish (Heterandria formosa). For acute toxicity, least killifish were exposed to 0, 25, 50, or 100μg/L of Cu for a 2-h duration and then tested for changes in shoaling behavior. The occurrence of copper acclimation was tested by pre-exposing fish to either 0 or 15μg/L of Cu for a 7-day duration, then exposing them to 25, 50, or 100μg/L of Cu to assess whether the two pre-exposure groups differed in their behavioral responses to high Cu levels. Behavioral responses were tested in a subdivided aquarium, with one side containing a group of conspecific fish and the other side the focal fish whose behavior was recorded for 3min. Shoaling was based on the proximity of the focal fish to the divider separating it from the group of conspecific fish. Acute copper exposure resulted in significant decreases in both the time to first shoaling and in the total amount of time spent shoaling. Experiments assessing the occurrence of copper acclimation using shoaling as the toxicity endpoint did not detect a difference between the pre-exposure groups. Overall, this study demonstrated that acute copper exposure can alter shoaling behavior. However, least killifish shoaling behavior appears to be relatively insensitive as an endpoint to assess copper acclimation. Springer Science+Business Media Dordrecht, 2013 Cu toxicity nationallicence Cu acclimation nationallicence Shoaling behavior nationallicence Fish behavioral effects nationallicence Vogt Sarah Department of Biology, University of Louisiana at Lafayette, Lafayette, LA, USA aut Billock Arlene Department of Biology, University of Louisiana at Lafayette, Lafayette, LA, USA aut Klerks Paul Department of Biology, University of Louisiana at Lafayette, Lafayette, LA, USA aut Water, Air, & Soil Pollution Springer Netherlands 224/7(2013-07-01), 1-8 0049-6979 224:7<1 2013 224 11270 https://doi.org/10.1007/s11270-013-1627-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11270-013-1627-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vogt Sarah Department of Biology, University of Louisiana at Lafayette, Lafayette, LA, USA aut NATIONALLICENCE 700 1- Billock Arlene Department of Biology, University of Louisiana at Lafayette, Lafayette, LA, USA aut NATIONALLICENCE 700 1- Klerks Paul Department of Biology, University of Louisiana at Lafayette, Lafayette, LA, USA aut NATIONALLICENCE 773 0- Water, Air, & Soil Pollution Springer Netherlands 224/7(2013-07-01), 1-8 0049-6979 224:7<1 2013 224 11270 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer