naa a22 4500 510750133 CHVBK 20180411083101.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s11270-013-1625-y doi (NATIONALLICENCE)springer-10.1007/s11270-013-1625-y A Novel Approach to Precipitation of Heavy Metals from Industrial Effluents and Single-Ion Solutions Using Bacterial Alkaline Phosphatase [Elektronische Daten] [Gouri Chaudhuri, Pritam Dey, Devjyoti Dalal, P. Venu-Babu, W. Thilagaraj] Enzymatic precipitation provides a novel cost-effective and eco-friendly method for remediation of heavy metals from different industrial effluents such as tannery, electroplating, dye industries, and many more. This study has paid attention to bacterial alkaline phosphatase (BAP) from Eschericia coli C90 which catalyzes para-nitrophenyl phosphate (pNPP) and produces inorganic phosphate (Pi) that helps in the precipitation of heavy metals as metalphosphates. The kinetic behavior of BAP with pNPP in Tris-HCl was studied for pH regimes 8, 8.5, 9, 9.5, 10, 10.5, and 11 in detail. The results showed that the maximum activity of the enzyme was at pH8.5 with an incubation period of 300min at 37°C. Based on the kinetic data, experiments were performed at pH8.5 and pH10 to precipitate Cr3+, Cr6+, Cd2+, Ni2+, and Co2+ from single-ion solutions (250 and 1,000ppm concentrations) as well as industrial effluents, and the amount of metal precipitated as metalphosphate was derived by determining the amount of metal reduced in the supernatant of the reactions employing atomic absorption spectrophotometer. The precipitation of metals from single-ion solutions at pH8.5 for 300min incubation period followed the order Cd2+ > Ni2+ > Cr3+ > Cr6+ > Co2+. In the experiments involving effluents from tannery and electroplating industries, precipitation of 35.1% of Cr6+, 77.80% of Ni2+, and 57.42% of Cd2+ was achieved from initial concentrations of 621, 97, and 122ppm, respectively. Springer Science+Business Media Dordrecht, 2013 p -Nitrophenyl phosphate nationallicence Bacterial alkaline phosphatase nationallicence Enzymatic precipitation nationallicence White biotechnology nationallicence Heavy metals nationallicence Industrial effluents nationallicence Chaudhuri Gouri Department of Biotechnology, School of Bioengineering, SRM University, 603203, Kattankulathur, Tamil Nadu, India aut Dey Pritam Department of Biotechnology, School of Bioengineering, SRM University, 603203, Kattankulathur, Tamil Nadu, India aut Dalal Devjyoti Department of Biotechnology, School of Bioengineering, SRM University, 603203, Kattankulathur, Tamil Nadu, India aut Venu-Babu P. Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, 400085, Mumbai, India aut Thilagaraj W. Department of Biotechnology, School of Bioengineering, SRM University, 603203, Kattankulathur, Tamil Nadu, India aut Water, Air, & Soil Pollution Springer Netherlands 224/7(2013-07-01), 1-11 0049-6979 224:7<1 2013 224 11270 https://doi.org/10.1007/s11270-013-1625-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11270-013-1625-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chaudhuri Gouri Department of Biotechnology, School of Bioengineering, SRM University, 603203, Kattankulathur, Tamil Nadu, India aut NATIONALLICENCE 700 1- Dey Pritam Department of Biotechnology, School of Bioengineering, SRM University, 603203, Kattankulathur, Tamil Nadu, India aut NATIONALLICENCE 700 1- Dalal Devjyoti Department of Biotechnology, School of Bioengineering, SRM University, 603203, Kattankulathur, Tamil Nadu, India aut NATIONALLICENCE 700 1- Venu-Babu P. Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, 400085, Mumbai, India aut NATIONALLICENCE 700 1- Thilagaraj W. Department of Biotechnology, School of Bioengineering, SRM University, 603203, Kattankulathur, Tamil Nadu, India aut NATIONALLICENCE 773 0- Water, Air, & Soil Pollution Springer Netherlands 224/7(2013-07-01), 1-11 0049-6979 224:7<1 2013 224 11270 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer