naa a22 4500 510750303 CHVBK 20180411083102.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s11270-013-1775-y doi (NATIONALLICENCE)springer-10.1007/s11270-013-1775-y Influence of Increasing Soil Copper Concentration on the Susceptibility of Phosphomonoesterase and Urease to Heat Disturbance [Elektronische Daten] [Adam Wightwick, Suzanne Reichman, Graeme Allinson, Neal Menzies] Long-term exposure to elevated copper (Cu) concentrations may affect the ability of soil microbes to withstand additional transient disturbances, such as heat. Bulk surface soil samples collected from three south east Australian locations were spiked with a series of Cu concentrations ranging from 0 to 1,000mg/kg. To determine the effect of increasing soil Cu concentrations on phosphomonoesterase and urease activity following a simulated heat disturbance, aliquots of each of the Cu-treated soils were exposed to 60°C for 24h (perturbed group), and phosphomonoesterase and urease activity measured after 1, 2 and 7days. Without heat disturbance, the Cu concentration causing significant inhibition of enzyme activity ranged from 50 to >1,000mg/kg added Cu for phosphomonoesterase activity and 500 to > 1,000mg/kg added Cu for urease activity. The Cu pre-exposure concentration increased the susceptibility of phosphomonoesterase activity but not urease activity to the heat disturbance. However, this did not follow a clear dose-response relationship and the effects were not lasting. The response differed according to soil type, with decreased resistance to heat found at concentrations ranging from 100mg/kg added Cu in the silty loam soil to >1,000mg/kg added Cu in the clay loam soil. The results from this study suggest that, at the concentrations of Cu typically reported in agricultural soils, Cu stress is unlikely to cause lasting alterations to the susceptibility of soil enzymes to a single moderate heat disturbance. Springer Science+Business Media Dordrecht, 2013 Copper nationallicence Soil nationallicence Enzyme activity nationallicence Functional stability nationallicence Resilience nationallicence Heat nationallicence Wightwick Adam School of Agriculture and Food Sciences, The University of Queensland, St Lucia, 4072, Brisbane, Queensland, Australia aut Reichman Suzanne Environment Protection Authority, Centre for Environmental Sciences, Macleod, 3085, Melbourne, Victoria, Australia aut Allinson Graeme Future Farming Systems Research Division, Department of Primary Industries Victoria Queenscliff Centre, PO Box 114, 3225, Queenscliff, Victoria, Australia aut Menzies Neal aut Water, Air, & Soil Pollution Springer Netherlands 224/12(2013-12-01), 1-12 0049-6979 224:12<1 2013 224 11270 https://doi.org/10.1007/s11270-013-1775-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11270-013-1775-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wightwick Adam School of Agriculture and Food Sciences, The University of Queensland, St Lucia, 4072, Brisbane, Queensland, Australia aut NATIONALLICENCE 700 1- Reichman Suzanne Environment Protection Authority, Centre for Environmental Sciences, Macleod, 3085, Melbourne, Victoria, Australia aut NATIONALLICENCE 700 1- Allinson Graeme Future Farming Systems Research Division, Department of Primary Industries Victoria Queenscliff Centre, PO Box 114, 3225, Queenscliff, Victoria, Australia aut NATIONALLICENCE 700 1- Menzies Neal aut NATIONALLICENCE 773 0- Water, Air, & Soil Pollution Springer Netherlands 224/12(2013-12-01), 1-12 0049-6979 224:12<1 2013 224 11270 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer