naa a22 4500 51075063X CHVBK 20180411083103.0 cr unu---uuuuu 180411e20130301xx s 000 0 eng 10.1007/s11270-013-1462-z doi (NATIONALLICENCE)springer-10.1007/s11270-013-1462-z A Single Metal, Metal Mixture, and Whole-Effluent Approach to Investigate Causes of Metal Mine Effluent Effects on Fathead Minnows ( Pimephales promelas ) [Elektronische Daten] [Jacob Ouellet, Monique Dubé, Som Niyogi] Although many studies have examined effects of metal mine effluents (MMEs) on receiving environments, few have compared the roles of individual and mixed metals relative to whole effluents. The objective of the present set of studies was to examine whether Cu, Ni, or Se, alone or in a mixture, causes comparable effects to those observed in fathead minnows (Pimephales promelas) exposed to an environmentally relevant MME (45% process water effluent [PWE]). Metal bioaccumulation, fathead minnow (FHM) morphometrics, and egg production were compared between treatments over a 21-day exposure. FHMs were exposed to similar waterborne concentrations and species of metals in single and mixed metal treatments relative to 45% PWE. FHMs were also exposed to similar concentrations of metals in single and mixed metal treatments relative to 45% PWE through the diet (Chironomus dilutus — a representative prey species). However, only FHMs exposed to 45% PWE had reduced egg production (60-80% less than controls). Our findings indicate that Cu, Ni, and Se exposures and bioaccumulation did not contribute to decreased reproductive output in FHMs under the conditions that were examined. We also found no evidence to believe that these metals were responsible for decreased egg production in PWE. It is therefore reasonable to suggest that these metals have limited potential to cause reproductive effects in MMEs with similar composition and water chemistry conditions. Overall, this study highlights the importance of examining single and mixed metal exposures prior to suggesting that adverse effects in fish exposed to MMEs occur due to bioaccumulation of metal(s). Springer Science+Business Media Dordrecht, 2013 Fathead minnow nationallicence Multi-trophic nationallicence Metal mining effluent nationallicence Single metals nationallicence Metal mixture nationallicence Reproductive bioassay nationallicence Ouellet Jacob Department of Biology, University of Saskatchewan, 112 Campus Drive, S7N 5E2, Saskatoon, Saskatchewan, Canada aut Dubé Monique Canadian Rivers Institute, University of New Brunswick, New Bruswick, Canada aut Niyogi Som Department of Biology, University of Saskatchewan, 112 Campus Drive, S7N 5E2, Saskatoon, Saskatchewan, Canada aut Water, Air, & Soil Pollution Springer Netherlands 224/3(2013-03-01), 1-19 0049-6979 224:3<1 2013 224 11270 https://doi.org/10.1007/s11270-013-1462-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11270-013-1462-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ouellet Jacob Department of Biology, University of Saskatchewan, 112 Campus Drive, S7N 5E2, Saskatoon, Saskatchewan, Canada aut NATIONALLICENCE 700 1- Dubé Monique Canadian Rivers Institute, University of New Brunswick, New Bruswick, Canada aut NATIONALLICENCE 700 1- Niyogi Som Department of Biology, University of Saskatchewan, 112 Campus Drive, S7N 5E2, Saskatoon, Saskatchewan, Canada aut NATIONALLICENCE 773 0- Water, Air, & Soil Pollution Springer Netherlands 224/3(2013-03-01), 1-19 0049-6979 224:3<1 2013 224 11270 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer