naa a22 4500 510750702 CHVBK 20180411083103.0 cr unu---uuuuu 180411e20130301xx s 000 0 eng 10.1007/s11270-013-1458-8 doi (NATIONALLICENCE)springer-10.1007/s11270-013-1458-8 Antioxidant Defenses and Trace Metal Bioaccumulation Capacity of Cymbula nigra (Gastropoda: Patellidae) [Elektronische Daten] [G. Rivera-Ingraham, G. Malanga, S. Puntarulo, A. Pérez, A. Ruiz-Tabares, M. Maestre, R. González-Aranda, F. Espinosa, J. García-Gómez] The present study deals with the effect of trace metals on the endangered limpet Cymbula nigra. The Bay of Algeciras (Strait of Gibraltar) was used as the study site. Important industrial activity takes place in the area, including frequent oil spills. However, it is home to important populations of C. nigra. The objective of this work was to determine if these animals were being affected at a subcellular level by the pollutants present in their environment and to analyze the trace metal concentrations in the animal's soft tissues. To determine the effects of water quality on the antioxidant activity and concentrations through field experimentation, a total of six sites were selected in Algeciras Bay, three located in the inner areas (environmentally degraded sites with higher levels of pollutants) and three in the outermost areas of the Bay. Stress associated to reactive oxygen species formation was assessed on digestive glands and gills as the enzymatic antioxidant activity of catalase (CAT), superoxide dismutase (SOD), and glutathione S-transferase (GST) and as the concentrations of lipid-soluble (α-tocopherol and β-carotene) and the water-soluble antioxidants (reduced and oxidized glutathione (GSH and GSSG)). Gills and digestive glands of those animals located in the inner areas of Algeciras Bay showed higher CAT activity values than those located in the outer areas. As a general pattern, we observed higher antioxidant activities and concentrations in digestive glands that in gills, suggesting the possibility that pollutants are mainly being incorporated by limpets through the food. As a general rule, larger animals showed greater concentrations of these compounds. Iron, zinc, and manganese, in this order, were present in the tissues at the highest concentrations. Chromium and manganese were found in significantly higher concentrations in those animals collected from the inner areas of the Bay. Through the present study, we provide the first data regarding the antioxidant defense levels and metal accumulation capacity of this species, and we reinforce the idea that this endangered species may be, in fact, relatively tolerant to degraded environments. Springer Science+Business Media Dordrecht, 2013 Cymbula nationallicence Trace metals nationallicence Limpet nationallicence ROS nationallicence Strait of Gibraltar nationallicence Subcellular stress nationallicence Rivera-Ingraham G. Laboratorio de Biología Marina, Departamento de Fisiología y Zoología, Universidad de Sevilla, Avenida Reina Mercedes 6, 41012, Sevilla, Spain aut Malanga G. Fisicoquímica-IBIMOL Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires, Argentina aut Puntarulo S. Fisicoquímica-IBIMOL Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires, Argentina aut Pérez A. Laboratorio de Ecología de Bentos Marino, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina aut Ruiz-Tabares A. Laboratorio de Biología Marina, Departamento de Fisiología y Zoología, Universidad de Sevilla, Avenida Reina Mercedes 6, 41012, Sevilla, Spain aut Maestre M. Laboratorio de Biología Marina, Departamento de Fisiología y Zoología, Universidad de Sevilla, Avenida Reina Mercedes 6, 41012, Sevilla, Spain aut González-Aranda R. Laboratorio de Biología Marina, Departamento de Fisiología y Zoología, Universidad de Sevilla, Avenida Reina Mercedes 6, 41012, Sevilla, Spain aut Espinosa F. Laboratorio de Biología Marina, Departamento de Fisiología y Zoología, Universidad de Sevilla, Avenida Reina Mercedes 6, 41012, Sevilla, Spain aut García-Gómez J. Laboratorio de Biología Marina, Departamento de Fisiología y Zoología, Universidad de Sevilla, Avenida Reina Mercedes 6, 41012, Sevilla, Spain aut Water, Air, & Soil Pollution Springer Netherlands 224/3(2013-03-01), 1-13 0049-6979 224:3<1 2013 224 11270 https://doi.org/10.1007/s11270-013-1458-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11270-013-1458-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rivera-Ingraham G. Laboratorio de Biología Marina, Departamento de Fisiología y Zoología, Universidad de Sevilla, Avenida Reina Mercedes 6, 41012, Sevilla, Spain aut NATIONALLICENCE 700 1- Malanga G. Fisicoquímica-IBIMOL Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Puntarulo S. Fisicoquímica-IBIMOL Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Pérez A. Laboratorio de Ecología de Bentos Marino, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Ruiz-Tabares A. Laboratorio de Biología Marina, Departamento de Fisiología y Zoología, Universidad de Sevilla, Avenida Reina Mercedes 6, 41012, Sevilla, Spain aut NATIONALLICENCE 700 1- Maestre M. Laboratorio de Biología Marina, Departamento de Fisiología y Zoología, Universidad de Sevilla, Avenida Reina Mercedes 6, 41012, Sevilla, Spain aut NATIONALLICENCE 700 1- González-Aranda R. Laboratorio de Biología Marina, Departamento de Fisiología y Zoología, Universidad de Sevilla, Avenida Reina Mercedes 6, 41012, Sevilla, Spain aut NATIONALLICENCE 700 1- Espinosa F. Laboratorio de Biología Marina, Departamento de Fisiología y Zoología, Universidad de Sevilla, Avenida Reina Mercedes 6, 41012, Sevilla, Spain aut NATIONALLICENCE 700 1- García-Gómez J. Laboratorio de Biología Marina, Departamento de Fisiología y Zoología, Universidad de Sevilla, Avenida Reina Mercedes 6, 41012, Sevilla, Spain aut NATIONALLICENCE 773 0- Water, Air, & Soil Pollution Springer Netherlands 224/3(2013-03-01), 1-13 0049-6979 224:3<1 2013 224 11270 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer