naa a22 4500 510750885 CHVBK 20180411083104.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s11270-013-1738-3 doi (NATIONALLICENCE)springer-10.1007/s11270-013-1738-3 Reduction of Net Sulfide Production Rate by Nitrate in Wastewater Bioreactors. Kinetics and Changes in the Microbial Community [Elektronische Daten] [D. Villahermosa, A. Corzo, J. González, M. Portillo, E. García-Robledo, S. Papaspyrou] Nitrate addition stimulated sulfide oxidation by increasing the activity of nitrate-reducing sulfide-oxidizing bacteria (NR-SOB), decreasing the concentration of dissolved H2S in the water phase and, consequently, its release to the atmosphere of a pilot-scale anaerobic bioreactor. The effect of four different concentrations of nitrate (0.12, 0.24, 0.50, and 1.00mM) was investigated for a period of 3days in relation to sulfide concentration in two bioreactors set up at Guadalete wastewater treatment plant (Jerez de la Frontera, Spain). Physicochemical variables were measured in water and air, and the activity of bacteria implicated in the sulfur and nitrogen cycles was analyzed in the biofilms and in the water phase of the bioreactors. Biofilms were a net source of sulfide for the water and gas phases (7.22 ± 5.3μmols−1) in the absence of nitrate dosing. Addition of nitrate resulted in a quick (within 3h) decrease of sulfide both in the water and atmospheric phases. Sulfide elimination efficiency in the water phase increased with nitrate concentrations following the Michaelis-Menten kinetics (K s = 0.63mM NO3 −). The end of nitrate addition resulted in a recovery or increase of initial net sulfide production in about 3h. Addition of nitrate increased the activity of NR-SOB and decreased the activity of sulfate-reducing bacteria. Results confirmed the role of NR-SOB on hydrogen sulfide consumption coupled with nitrate reduction and sulfate recycling, revealing Sulfurimonas denitrificans and Paracoccus denitrificans as NR-SOB of great importance in this process. Springer Science+Business Media Dordrecht, 2013 Sulfide removal nationallicence Autotrophic nitrate reduction nationallicence Wastewater nationallicence Biofilm nationallicence Kinetics nationallicence Microbial community nationallicence Villahermosa D. Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, Pol. Río San Pedro s/n, 11510, Puerto Real, Cádiz, Spain aut Corzo A. Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, Pol. Río San Pedro s/n, 11510, Puerto Real, Cádiz, Spain aut González J. Instituto de Recursos Naturales y Agrobiología, IRNAS-CSIC, Avda. Reina Mercedes 10, 41012, Sevilla, Spain aut Portillo M. Instituto de Recursos Naturales y Agrobiología, IRNAS-CSIC, Avda. Reina Mercedes 10, 41012, Sevilla, Spain aut García-Robledo E. Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, Pol. Río San Pedro s/n, 11510, Puerto Real, Cádiz, Spain aut Papaspyrou S. Unidad asociada de Oceanografía Interdisciplinar, Instituto de Ciencias Marinas de Andalucía, ICMAN-CSIC, Pol. Río San Pedro s/n, 11510, Puerto Real, Cádiz, Spain aut Water, Air, & Soil Pollution Springer Netherlands 224/10(2013-10-01), 1-15 0049-6979 224:10<1 2013 224 11270 https://doi.org/10.1007/s11270-013-1738-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11270-013-1738-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Villahermosa D. Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, Pol. Río San Pedro s/n, 11510, Puerto Real, Cádiz, Spain aut NATIONALLICENCE 700 1- Corzo A. Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, Pol. Río San Pedro s/n, 11510, Puerto Real, Cádiz, Spain aut NATIONALLICENCE 700 1- González J. Instituto de Recursos Naturales y Agrobiología, IRNAS-CSIC, Avda. Reina Mercedes 10, 41012, Sevilla, Spain aut NATIONALLICENCE 700 1- Portillo M. Instituto de Recursos Naturales y Agrobiología, IRNAS-CSIC, Avda. Reina Mercedes 10, 41012, Sevilla, Spain aut NATIONALLICENCE 700 1- García-Robledo E. Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, Pol. Río San Pedro s/n, 11510, Puerto Real, Cádiz, Spain aut NATIONALLICENCE 700 1- Papaspyrou S. Unidad asociada de Oceanografía Interdisciplinar, Instituto de Ciencias Marinas de Andalucía, ICMAN-CSIC, Pol. Río San Pedro s/n, 11510, Puerto Real, Cádiz, Spain aut NATIONALLICENCE 773 0- Water, Air, & Soil Pollution Springer Netherlands 224/10(2013-10-01), 1-15 0049-6979 224:10<1 2013 224 11270 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer