naa a22 4500 510751172 CHVBK 20180411083105.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s11270-013-1457-9 doi (NATIONALLICENCE)springer-10.1007/s11270-013-1457-9 Structural Characterization and Property Analysis of Surface-Active Substance Accumulated During Biodegradation of Hydrophobic α-Pinene by Pseudomonas fluorescens [Elektronische Daten] [Zhuo-Wei Cheng, Jian-Meng Chen, Jian-ming Yu, Shan-Shan Li, Yi-Feng Jiang] The connection between hydrocarbon biodegradation and surface-active substance production has attracted great interest in recent years. Pseudomonas fluorescens PT, isolated from a biotrickling filter, was not only able to degrade α-pinene but also to use it as a carbon source to produce a surface-active substance. Response surface methodology analysis showed that the optimal medium composition was K+, 69.8; Mn2+, 65.1; and NH4 +, 482.5mg L−1, at which the surface tension of the medium was reduced to 40.7 mN m−1 after 36h. Based on compositional analysis and information on α-pinene metabolism, the purified compound was identified as perilla acid. The surface-active properties of the purified compound were more stable than those of a synthetic surfactant, and it had lower ecological toxicity to Chlorella vulgaris. The naphthalene solubility and mass transfer of α-pinene were enhanced almost twofold by the surface-active substance (at its critical micelle concentration). The results suggested that the PT strain may be promising for generating surface-active substances with improved physiochemical properties for a wide range of applications in environmental remediation. Springer Science+Business Media Dordrecht, 2013 Surface-active substance nationallicence α-Pinene biodegradation nationallicence Stability of surface-active substance nationallicence Solubility of surface-active substance nationallicence Cheng Zhuo-Wei Zhejiang University of Technology, 310032, Hangzhou, China aut Chen Jian-Meng Zhejiang University of Technology, 310032, Hangzhou, China aut Yu Jian-ming Zhejiang University of Technology, 310032, Hangzhou, China aut Li Shan-Shan Zhejiang University of Technology, 310032, Hangzhou, China aut Jiang Yi-Feng Zhejiang University of Technology, 310032, Hangzhou, China aut Water, Air, & Soil Pollution Springer Netherlands 224/4(2013-04-01), 1-12 0049-6979 224:4<1 2013 224 11270 https://doi.org/10.1007/s11270-013-1457-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11270-013-1457-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cheng Zhuo-Wei Zhejiang University of Technology, 310032, Hangzhou, China aut NATIONALLICENCE 700 1- Chen Jian-Meng Zhejiang University of Technology, 310032, Hangzhou, China aut NATIONALLICENCE 700 1- Yu Jian-ming Zhejiang University of Technology, 310032, Hangzhou, China aut NATIONALLICENCE 700 1- Li Shan-Shan Zhejiang University of Technology, 310032, Hangzhou, China aut NATIONALLICENCE 700 1- Jiang Yi-Feng Zhejiang University of Technology, 310032, Hangzhou, China aut NATIONALLICENCE 773 0- Water, Air, & Soil Pollution Springer Netherlands 224/4(2013-04-01), 1-12 0049-6979 224:4<1 2013 224 11270 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer