naa a22 4500 510751253 CHVBK 20180411083105.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s11270-013-1490-8 doi (NATIONALLICENCE)springer-10.1007/s11270-013-1490-8 A Novel Autotrophic Bacterium Isolated from an Engineered Wetland System Links Nitrate-Coupled Iron Oxidation to the Removal of As, Zn and S [Elektronische Daten] [Al Mattes, Douglas Gould, Marcus Taupp, Susan Glasauer] A neutrophilic, autotrophic bacterium that couples iron oxidation to nitrate reduction (iron-oxidizing bacteria [IOB]) under anoxic conditions was isolated from a working bioremediation site in Trail, British Columbia. The site was designed and developed primarily to treat high concentrations of Zn and As that originate from capped industrial landfill sites. The system consisted of two upflow biochemical reactor cells (BCR) followed by three vegetated wetland polishing cells with sub-surface flow and a holding pond. During a 5-year period (2003-2007), the system treated more than 19,100m3 of contaminated water, removing and sequestering more than 10,700kg of As, Zn and sulfate at average input water concentrations of: As, 58.6mg l−1 (±39.9mg l−1); Zn, 51.9mg l−1 (±35.4mg l−1) and SO4 2−, 781.5mg l−1 (±287.8mg l−1). The bacterium was isolated in order to better understand the mechanisms underlying the consistent As removal that took place in the system. Analysis using Basic Local Alignment Search Tool (BLAST) database showed that the closest homologies are to Candidatus accumulibacterphosphatis (95% homology), Dechloromonas aromatica (94%), and Sideroxydans lithotrophicus ES-1 (92%) Within the BCR cells, the IOB oxidized Fe2+ generated by iron-reducing bacteria (IRB); the source of the iron was most likely biosolids and coatings of iron oxide on locally available sand used in the matrix. We have provisionally designated the novel bacterium as TR1. Springer Science+Business Media Dordrecht, 2013 Arsenic nationallicence Zinc nationallicence Iron-oxidizing bacteria nationallicence Sulfate reduction nationallicence Wetland nationallicence Mattes Al School of Environmental Sciences, University of Guelph, Guelph, ON, Canada aut Gould Douglas Mining and Mineral Sciences Laboratory, Natural Resources Canada, 555 Booth Street, Ottawa, ON, Canada aut Taupp Marcus Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada aut Glasauer Susan School of Environmental Sciences, University of Guelph, Guelph, ON, Canada aut Water, Air, & Soil Pollution Springer Netherlands 224/4(2013-04-01), 1-15 0049-6979 224:4<1 2013 224 11270 https://doi.org/10.1007/s11270-013-1490-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11270-013-1490-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mattes Al School of Environmental Sciences, University of Guelph, Guelph, ON, Canada aut NATIONALLICENCE 700 1- Gould Douglas Mining and Mineral Sciences Laboratory, Natural Resources Canada, 555 Booth Street, Ottawa, ON, Canada aut NATIONALLICENCE 700 1- Taupp Marcus Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada aut NATIONALLICENCE 700 1- Glasauer Susan School of Environmental Sciences, University of Guelph, Guelph, ON, Canada aut NATIONALLICENCE 773 0- Water, Air, & Soil Pollution Springer Netherlands 224/4(2013-04-01), 1-15 0049-6979 224:4<1 2013 224 11270 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer