naa a22 4500 510751504 CHVBK 20180411083106.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s11270-013-1523-3 doi (NATIONALLICENCE)springer-10.1007/s11270-013-1523-3 Assessment of the Genotoxicity of Two Agricultural Residues After Processing by Diplopods Using the Allium cepa Assay [Elektronische Daten] [Cintya Christofoletti, Janaína Pedro-Escher, Carmem Fontanetti] Agroindustrial by-products and residues from treatment of sewage sludge have been recently recycled as soil amendments. This study was aimed at assessing toxic potential of biosolid, obtained from a sewage treatment plant (STP), vinasse, a by-product of the sugar cane industry, and a combination of both residues using Allium cepa assay. Bioprocessing of these samples by a terrestrial invertebrate (diplopod Rhinocricus padbergi) was also examined. Bioassay assembly followed standards of the Brazilian legislation for disposal of these residues. After adding residues, 20 diplopods were placed in each terrarium, where they remained for 30days. Chemical analysis and the A. cepa assay were conducted before and after bioprocessing by diplopods. At the end of the bioassay, there was a decrease in arsenic and mercury. For the remaining metals, accumulation and/or bioavailability varied in all samples but suggested bioprocessing by animals. The A. cepa test revealed genotoxic effects characterized by different chromosome aberrations. Micronuclei and chromosome breaks on meristematic cells and F1 cells with micronuclei were examined to assess mutagenicity of samples. After 30days, the genotoxic effects were significantly reduced in the soil + biosolid and soil + biosolid + vinasse groups as well as the mutagenic effects in the soil + biosolid + vinasse group. Similar to vermicomposting, bioprocessing of residues by diplopods can be a feasible alternative and used prior to application in crops to improve degraded soils and/or city dumps. Based on our findings, further studies are needed to adequately dispose of these residues in the environment. Springer Science+Business Media Dordrecht, 2013 Biosolid nationallicence Sugar cane vinasse nationallicence Diplopods nationallicence Genotoxicity nationallicence Mutagenicity nationallicence Christofoletti Cintya UNESP (São Paulo State University), Av. 24-A, no. 1515, CP 199, 13506-900, Rio Claro, SP, Brazil aut Pedro-Escher Janaína UNESP (São Paulo State University), Av. 24-A, no. 1515, CP 199, 13506-900, Rio Claro, SP, Brazil aut Fontanetti Carmem UNESP (São Paulo State University), Av. 24-A, no. 1515, CP 199, 13506-900, Rio Claro, SP, Brazil aut Water, Air, & Soil Pollution Springer Netherlands 224/4(2013-04-01), 1-14 0049-6979 224:4<1 2013 224 11270 https://doi.org/10.1007/s11270-013-1523-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11270-013-1523-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Christofoletti Cintya UNESP (São Paulo State University), Av. 24-A, no. 1515, CP 199, 13506-900, Rio Claro, SP, Brazil aut NATIONALLICENCE 700 1- Pedro-Escher Janaína UNESP (São Paulo State University), Av. 24-A, no. 1515, CP 199, 13506-900, Rio Claro, SP, Brazil aut NATIONALLICENCE 700 1- Fontanetti Carmem UNESP (São Paulo State University), Av. 24-A, no. 1515, CP 199, 13506-900, Rio Claro, SP, Brazil aut NATIONALLICENCE 773 0- Water, Air, & Soil Pollution Springer Netherlands 224/4(2013-04-01), 1-14 0049-6979 224:4<1 2013 224 11270 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer