naa a22 4500 510751571 CHVBK 20180411083106.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s11270-013-1646-6 doi (NATIONALLICENCE)springer-10.1007/s11270-013-1646-6 A Pretreatment Method for Analysing Albendazole by HPLC in Plant Material [Elektronische Daten] [D. Marciocha, J. Kalka, J. Turek-Szytow, J. Surmacz-Górska] Albendazole (ALB) belongs to a group of benzimidazoles—classified as antiparasitic pharmaceuticals. Its widespread application results in the presence of this pharmaceutical in natural environment (water and soil). In this paper a suitable pretreatment method was established including sampling, freeze-drying and extraction. Vicia faba was used as model organism. ALB accumulation by plant tissues was observed in hydroponic culture as well as in soil. The range of pharmaceutical concentrations was 1.7 × 10−5mol/L (in hydroponic culture) and 1.7 × 10−5 to 1.7 × 10−4mol/kg air dry soil (in soil). Observations were conducted for 14days. After this time biological material was freeze-dried and after homogenization, dimethyl sulfoxide (DMSO) extraction was performed. The recovery of ALB for the roots was 93% while for the shoots 86%. After cleaning, the samples were subjected to further analysis by HPLC system. Phosphate buffer and acetonitrile (50:50) were used as a mobile phase. Drug retention time was 6.3min. Results obtained in this experiment indicate higher drug accumulation in roots rather than in the hypocotyl part of the plant, cultivated both in soil and in hydroponic culture. The Author(s), 2013 Albendazole nationallicence Plant nationallicence HPLC nationallicence Sample preparation nationallicence Marciocha D. Silesian University of Technology, Akademicka 2, 44-100, Gliwice, Poland aut Kalka J. Silesian University of Technology, Akademicka 2, 44-100, Gliwice, Poland aut Turek-Szytow J. Silesian University of Technology, Akademicka 2, 44-100, Gliwice, Poland aut Surmacz-Górska J. Silesian University of Technology, Akademicka 2, 44-100, Gliwice, Poland aut Water, Air, & Soil Pollution Springer Netherlands 224/8(2013-08-01), 1-8 0049-6979 224:8<1 2013 224 11270 https://doi.org/10.1007/s11270-013-1646-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11270-013-1646-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Marciocha D. Silesian University of Technology, Akademicka 2, 44-100, Gliwice, Poland aut NATIONALLICENCE 700 1- Kalka J. Silesian University of Technology, Akademicka 2, 44-100, Gliwice, Poland aut NATIONALLICENCE 700 1- Turek-Szytow J. Silesian University of Technology, Akademicka 2, 44-100, Gliwice, Poland aut NATIONALLICENCE 700 1- Surmacz-Górska J. Silesian University of Technology, Akademicka 2, 44-100, Gliwice, Poland aut NATIONALLICENCE 773 0- Water, Air, & Soil Pollution Springer Netherlands 224/8(2013-08-01), 1-8 0049-6979 224:8<1 2013 224 11270 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer