naa a22 4500 510751598 CHVBK 20180411083106.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s11270-013-1645-7 doi (NATIONALLICENCE)springer-10.1007/s11270-013-1645-7 Effects of Pseudomonas fluorescens Seed Bioinoculation on Heavy Metal Accumulation for Mirabilis jalapa Phytoextraction in Smelter-Contaminated Soil [Elektronische Daten] [M. Petriccione, D. Di Patre, P. Ferrante, S. Papa, G. Bartoli, A. Fioretto, M. Scortichini] Some Pseudomonas fluorescens strains, consistently isolated from the rhizosphere of wild plants grown in a soil that was highly polluted with illegal waste of smelter residues, were utilised for Mirabilis jalapa seed bioinoculation to verify their effects on seed germination and on promoting a higher heavy metal accumulation in the plant rhizosphere and/or uptake in the leaves. The high content of heavy metals in the soil induced a decrease in either the leaf dry weight or photosynthetic pigment concentration during all vegetative phase of M. jalapa. Bioinoculation with P. fluorescens strains significantly increased the germination of seeds and the root length in the contaminated soil. In some bacterial strain/seed combination, bioinoculation significantly increased the accumulation of heavy metals in M. jalapa rhizosphere. For Cd, the concentration of this metal in the rhizospheres of bioinoculated plants ranged from 270 to 910μgg−1 of dry weight compared with 200μgg−1 of dry weight for the non-coated plants. Two P. fluorescens strains, AA27 and MO49, which were isolated from Artemisia annua and Melilotus officinalis, respectively, induced a significantly higher rhizosphere availability also for Cr, Cu, Ni and Zn. However, despite the relevant accumulation of the heavy metals in the plant rhizosphere, generally the metal uptake into the leaves was rather low. Both analysis of variance and principal component analysis confirmed this finding. However, one P. fluorescens strain, CD1, which was isolated from the multi-metal accumulator Cynodon dactylon, significantly promoted the M. jalapa leaf uptake for Cr, Cu and Zn. The plant metal uptake assessment, confirmed the per se capability of M. jalapa to effectively uptake Cd (30%) and Cu (12.72%) from the rhizosphere to the leaves, whereas the uptake for the other metals was low: Ni (2.66%), Zn (2.46%), Cr (1.75%), Pb (0.73%). Springer Science+Business Media Dordrecht, 2013 Bacteria-assisted phytoremediation nationallicence Soil pollution nationallicence PGPR nationallicence Fluorescent pseudomonads nationallicence Hyperaccumulator plants nationallicence Petriccione M. Consiglio per la Ricerca e la Sperimentazione in Agricoltura — Fruit Trees Unit Research, Via Torrino 3, 81100, Caserta, Italy aut Di Patre D. Consiglio per la Ricerca e la Sperimentazione in Agricoltura — Fruit Trees Unit Research, Via Torrino 3, 81100, Caserta, Italy aut Ferrante P. Consiglio per la Ricerca e la Sperimentazione in Agricoltura — Fruit Crops Research Centre, Via di Fioranello 52, 00134, Roma, Italy aut Papa S. Life Science Department, Second University of Naples, Via Vivaldi 43, 81100, Caserta, Italy aut Bartoli G. Life Science Department, Second University of Naples, Via Vivaldi 43, 81100, Caserta, Italy aut Fioretto A. Life Science Department, Second University of Naples, Via Vivaldi 43, 81100, Caserta, Italy aut Scortichini M. Consiglio per la Ricerca e la Sperimentazione in Agricoltura — Fruit Trees Unit Research, Via Torrino 3, 81100, Caserta, Italy aut Water, Air, & Soil Pollution Springer Netherlands 224/8(2013-08-01), 1-17 0049-6979 224:8<1 2013 224 11270 https://doi.org/10.1007/s11270-013-1645-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11270-013-1645-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Petriccione M. Consiglio per la Ricerca e la Sperimentazione in Agricoltura — Fruit Trees Unit Research, Via Torrino 3, 81100, Caserta, Italy aut NATIONALLICENCE 700 1- Di Patre D. Consiglio per la Ricerca e la Sperimentazione in Agricoltura — Fruit Trees Unit Research, Via Torrino 3, 81100, Caserta, Italy aut NATIONALLICENCE 700 1- Ferrante P. Consiglio per la Ricerca e la Sperimentazione in Agricoltura — Fruit Crops Research Centre, Via di Fioranello 52, 00134, Roma, Italy aut NATIONALLICENCE 700 1- Papa S. Life Science Department, Second University of Naples, Via Vivaldi 43, 81100, Caserta, Italy aut NATIONALLICENCE 700 1- Bartoli G. Life Science Department, Second University of Naples, Via Vivaldi 43, 81100, Caserta, Italy aut NATIONALLICENCE 700 1- Fioretto A. Life Science Department, Second University of Naples, Via Vivaldi 43, 81100, Caserta, Italy aut NATIONALLICENCE 700 1- Scortichini M. Consiglio per la Ricerca e la Sperimentazione in Agricoltura — Fruit Trees Unit Research, Via Torrino 3, 81100, Caserta, Italy aut NATIONALLICENCE 773 0- Water, Air, & Soil Pollution Springer Netherlands 224/8(2013-08-01), 1-17 0049-6979 224:8<1 2013 224 11270 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer