naa a22 4500 510751997 CHVBK 20180411083108.0 cr unu---uuuuu 180411e20130501xx s 000 0 eng 10.1007/s11270-013-1554-9 doi (NATIONALLICENCE)springer-10.1007/s11270-013-1554-9 Anaerobic Degradation of Protein: Simplified Kinetic Modelling and Microbial Dynamics [Elektronische Daten] [Giovana Tommaso, Mercia Domingues, Rogers Ribeiro, Maria Varesche, Marcelo Zaiat, Eugenio Foresti] Data on the influence of substrate composition on the anaerobic degradation of peptone in a bench-scale packed-bed reactor are presented and discussed. The experiments were conducted in a horizontal-flow anaerobic immobilised biomass reactor operated with a hydraulic detention time of 4h. Peptone was the sole carbon source in the first experiment (E1). In the second experiment (E2), the reactor was fed with peptone and carbohydrates, and in the third experiment (E3), lipids were also added. At end of each experiment, the samples were collected to obtain spatial profiles of the substrates and intermediary metabolites. A modified first-order kinetic expression fits well with the chemical oxygen demand data, allowing kinetic parameter inference in both E1 and E2. The presence of lipids in the E3 influent clearly disturbed the equilibrium of the process, which could be better represented by two first-order kinetic expressions in series. A kinetic model of irreversible first-order reactions (in series and in parallel) with two intermediate products was proposed for representing the entire process. Several modifications of the metabolic routes were clearly represented by the values of the model parameters. It was also possible to conclude that the adsorption of lipids in the fixed bed caused a decrease in the consumption rate of proteins and acetate. Microscopy examinations and fluorescence in situ hybridisation analyses corroborated the conclusions from the kinetic study. The frequencies of the microorganisms changed as the substrate composition was modified, indicating the capability of the microorganisms to adapt. Springer Science+Business Media Dordrecht, 2013 Anaerobic process nationallicence Protein degradation nationallicence Anaerobic reactors nationallicence Kinetic modelling nationallicence HAIB reactor nationallicence Tommaso Giovana Laboratório de Biotecnologia Ambiental (LBA), Departamento de Engenharia de Alimentos (ZEA), Faculdade de Zootecnia e Engenharia de Alimentos (FZEA), Universidade de São Paulo (USP), Rua Duque de Caxias Norte 225, 13635-900, Pirassununga, SP, Brazil aut Domingues Mercia Laboratório de Processos Biológicos (LPB), Centro de Pesquisa, Desenvolvimento e Inovação em Engenharia Ambiental, Escola de Engenharia de São Carlos (EESC), Universidade de São Paulo (USP), Engenharia Ambiental, Bloco 4-F, Av. João Dagnone, 1100, Santa Angelina, 13.563-120, São Carlos, SP, Brazil aut Ribeiro Rogers Laboratório de Biotecnologia Ambiental (LBA), Departamento de Engenharia de Alimentos (ZEA), Faculdade de Zootecnia e Engenharia de Alimentos (FZEA), Universidade de São Paulo (USP), Rua Duque de Caxias Norte 225, 13635-900, Pirassununga, SP, Brazil aut Varesche Maria Laboratório de Processos Biológicos (LPB), Centro de Pesquisa, Desenvolvimento e Inovação em Engenharia Ambiental, Escola de Engenharia de São Carlos (EESC), Universidade de São Paulo (USP), Engenharia Ambiental, Bloco 4-F, Av. João Dagnone, 1100, Santa Angelina, 13.563-120, São Carlos, SP, Brazil aut Zaiat Marcelo Laboratório de Processos Biológicos (LPB), Centro de Pesquisa, Desenvolvimento e Inovação em Engenharia Ambiental, Escola de Engenharia de São Carlos (EESC), Universidade de São Paulo (USP), Engenharia Ambiental, Bloco 4-F, Av. João Dagnone, 1100, Santa Angelina, 13.563-120, São Carlos, SP, Brazil aut Foresti Eugenio Laboratório de Processos Biológicos (LPB), Centro de Pesquisa, Desenvolvimento e Inovação em Engenharia Ambiental, Escola de Engenharia de São Carlos (EESC), Universidade de São Paulo (USP), Engenharia Ambiental, Bloco 4-F, Av. João Dagnone, 1100, Santa Angelina, 13.563-120, São Carlos, SP, Brazil aut Water, Air, & Soil Pollution Springer Netherlands 224/5(2013-05-01), 1-12 0049-6979 224:5<1 2013 224 11270 https://doi.org/10.1007/s11270-013-1554-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11270-013-1554-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tommaso Giovana Laboratório de Biotecnologia Ambiental (LBA), Departamento de Engenharia de Alimentos (ZEA), Faculdade de Zootecnia e Engenharia de Alimentos (FZEA), Universidade de São Paulo (USP), Rua Duque de Caxias Norte 225, 13635-900, Pirassununga, SP, Brazil aut NATIONALLICENCE 700 1- Domingues Mercia Laboratório de Processos Biológicos (LPB), Centro de Pesquisa, Desenvolvimento e Inovação em Engenharia Ambiental, Escola de Engenharia de São Carlos (EESC), Universidade de São Paulo (USP), Engenharia Ambiental, Bloco 4-F, Av. João Dagnone, 1100, Santa Angelina, 13.563-120, São Carlos, SP, Brazil aut NATIONALLICENCE 700 1- Ribeiro Rogers Laboratório de Biotecnologia Ambiental (LBA), Departamento de Engenharia de Alimentos (ZEA), Faculdade de Zootecnia e Engenharia de Alimentos (FZEA), Universidade de São Paulo (USP), Rua Duque de Caxias Norte 225, 13635-900, Pirassununga, SP, Brazil aut NATIONALLICENCE 700 1- Varesche Maria Laboratório de Processos Biológicos (LPB), Centro de Pesquisa, Desenvolvimento e Inovação em Engenharia Ambiental, Escola de Engenharia de São Carlos (EESC), Universidade de São Paulo (USP), Engenharia Ambiental, Bloco 4-F, Av. João Dagnone, 1100, Santa Angelina, 13.563-120, São Carlos, SP, Brazil aut NATIONALLICENCE 700 1- Zaiat Marcelo Laboratório de Processos Biológicos (LPB), Centro de Pesquisa, Desenvolvimento e Inovação em Engenharia Ambiental, Escola de Engenharia de São Carlos (EESC), Universidade de São Paulo (USP), Engenharia Ambiental, Bloco 4-F, Av. João Dagnone, 1100, Santa Angelina, 13.563-120, São Carlos, SP, Brazil aut NATIONALLICENCE 700 1- Foresti Eugenio Laboratório de Processos Biológicos (LPB), Centro de Pesquisa, Desenvolvimento e Inovação em Engenharia Ambiental, Escola de Engenharia de São Carlos (EESC), Universidade de São Paulo (USP), Engenharia Ambiental, Bloco 4-F, Av. João Dagnone, 1100, Santa Angelina, 13.563-120, São Carlos, SP, Brazil aut NATIONALLICENCE 773 0- Water, Air, & Soil Pollution Springer Netherlands 224/5(2013-05-01), 1-12 0049-6979 224:5<1 2013 224 11270 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer