naa a22 4500 510752233 CHVBK 20180411083108.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s11270-012-1411-2 doi (NATIONALLICENCE)springer-10.1007/s11270-012-1411-2 Assessing Limitations for PAH Biodegradation in Long-Term Contaminated Soils Using Bioaccessibility Assays [Elektronische Daten] [Nagissa Mahmoudi, Greg Slater, Albert Juhasz] Polycyclic aromatic hydrocarbons (PAHs) are generated by a range of industrial processes including petroleum and gas production and are often found in high concentrations at industrial sites. Once PAHs enter the environment, the predominant mechanisms for removal are biological via microbial activity. However, PAHs have the potential to partition onto soil organic matter thereby decreasing their bioavailability to microorganisms and limiting their degradation. This explanation was felt to be the reason for a lack of evidence of PAH biodegradation in a study of long-term contaminated soils. To test the hypothesis that bioavailability was a limiting factor for biodegradation in theses soils, PAH bioavailability was determined using nonexhaustive extraction (propanol, butanol, hydrooxypropyl-β-cyclodextrin) and oxidation (persulfate) methodologies designed to determine the fraction of contaminants within soil which are available for biological uptake. The assays gave varying results for each soil, and no specific trends across all soils were observed. PAH bioaccessibility, derived from the HP-β-CD assay which has been the most extensively tested in the literature, was estimated to be between 0 and 10% for most soils, with the exception of pyrene, indicating that a large fraction of the soil-borne PAHs at the site are not available to microorganisms and that bioavailability limitations may be a primary cause for the lack of observed biodegradation at this site. These results highlight the importance of bioavailability to PAH degradation as well as the relevance of utilizing an assay that has been evaluated across many soil conditions and parameters. Springer Science+Business Media Dordrecht, 2013 Biodegradation nationallicence Bioaccessibility nationallicence PAHs nationallicence Bioremediation nationallicence Mahmoudi Nagissa School of Geography and Earth Sciences, McMaster University, 1280 Main St. W, L8S 4K1, Hamilton, ON, Canada aut Slater Greg School of Geography and Earth Sciences, McMaster University, 1280 Main St. W, L8S 4K1, Hamilton, ON, Canada aut Juhasz Albert Centre for Environmental Risk Assessment and Remediation, University of South Australia, Mawson Lakes Campus, 5095, Adelaide, SA, Australia aut Water, Air, & Soil Pollution Springer Netherlands 224/2(2013-02-01), 1-11 0049-6979 224:2<1 2013 224 11270 https://doi.org/10.1007/s11270-012-1411-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11270-012-1411-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mahmoudi Nagissa School of Geography and Earth Sciences, McMaster University, 1280 Main St. W, L8S 4K1, Hamilton, ON, Canada aut NATIONALLICENCE 700 1- Slater Greg School of Geography and Earth Sciences, McMaster University, 1280 Main St. W, L8S 4K1, Hamilton, ON, Canada aut NATIONALLICENCE 700 1- Juhasz Albert Centre for Environmental Risk Assessment and Remediation, University of South Australia, Mawson Lakes Campus, 5095, Adelaide, SA, Australia aut NATIONALLICENCE 773 0- Water, Air, & Soil Pollution Springer Netherlands 224/2(2013-02-01), 1-11 0049-6979 224:2<1 2013 224 11270 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer