naa a22 4500 510752640 CHVBK 20180411083110.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s11270-012-1364-5 doi (NATIONALLICENCE)springer-10.1007/s11270-012-1364-5 Estimating In Situ Biodegradation Rates of Petroleum Hydrocarbons and Microbial Population Dynamics by Performing Single-Well Push-Pull Tests in a Fractured Bedrock Aquifer [Elektronische Daten] [Yunchul Cho, Kyungjin Han, Namhee Kim, Sunhwa Park, Young Kim] The single-well push-pull test (SWPPT) was adapted to quantify in situ aerobic respiration and denitrification rates and to assess microbial population dynamics in a petroleum-contaminated fractured bedrock aquifer. Among three test wells, significant dissolved oxygen (DO) consumption was observed only in one well, with average zero- and first-order rate coefficients of 0.32 ± 0.63 and 7.07 ± 13.85mmol L−1day−1, respectively. Of the four test wells, significant NO3 − consumption was noted in three wells. The average zero- and first-order rate coefficients were 2.87 ± 2.21 and 11.83 ± 7.99mmol L−1day−1, respectively. These results indicate that NO3 − was more effectively consumed within this fractured bedrock aquifer. Significant DO or NO3 − (electron acceptors (EAs)) consumption, the limited contribution of Fe(II) to overall EAs consumption, the production of dissolved CO2 during aerobic respiration and denitrification tests, and N2O production strongly suggest that the EAs consumption was largely due to microbial activity. Detection of Variovorax paradox, benzene-degrading culture, and 28 novel microbial species after the addition of O2 or NO3 − suggests that EA injection into a fractured rock aquifer may stimulate aerobic or denitrifying petroleum-degrading microbes. Therefore, SWPPT may be useful for quantifying in situ aerobic respiration and denitrification rates and for assessing microbial population dynamics in petroleum-contaminated fractured bedrock aquifers. Springer Science+Business Media Dordrecht, 2012 Push-pull test nationallicence Toluene nationallicence Biodegradation rate nationallicence Fractured bedrock aquifer nationallicence SWPPT : Single-well push-pull test nationallicence BTEX : Benzene, toluene, ethylbenzene, and xylene nationallicence ED : Electron donor nationallicence EA : Electron acceptor nationallicence CS : Carbon source nationallicence Cho Yunchul Department of Environmental Engineering, Daejeon University (Former: Research Institute for Environmental Technology and Sustainable Development, Korea University, Seoul, South Korea), Daejeon, South Korea aut Han Kyungjin Department of Environmental Engineering, Korea University, Choongnam, South Korea aut Kim Namhee SK Innovation Co. Ltd., Seoul, South Korea aut Park Sunhwa National Institute of Environmental Research, Incheon, South Korea aut Kim Young Department of Environmental Engineering, Korea University, Choongnam, South Korea aut Water, Air, & Soil Pollution Springer Netherlands 224/2(2013-02-01), 1-14 0049-6979 224:2<1 2013 224 11270 https://doi.org/10.1007/s11270-012-1364-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11270-012-1364-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cho Yunchul Department of Environmental Engineering, Daejeon University (Former: Research Institute for Environmental Technology and Sustainable Development, Korea University, Seoul, South Korea), Daejeon, South Korea aut NATIONALLICENCE 700 1- Han Kyungjin Department of Environmental Engineering, Korea University, Choongnam, South Korea aut NATIONALLICENCE 700 1- Kim Namhee SK Innovation Co. Ltd., Seoul, South Korea aut NATIONALLICENCE 700 1- Park Sunhwa National Institute of Environmental Research, Incheon, South Korea aut NATIONALLICENCE 700 1- Kim Young Department of Environmental Engineering, Korea University, Choongnam, South Korea aut NATIONALLICENCE 773 0- Water, Air, & Soil Pollution Springer Netherlands 224/2(2013-02-01), 1-14 0049-6979 224:2<1 2013 224 11270 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer