naa a22 4500 510752675 CHVBK 20180411083110.0 cr unu---uuuuu 180411e20131101xx s 000 0 eng 10.1007/s11270-013-1772-1 doi (NATIONALLICENCE)springer-10.1007/s11270-013-1772-1 Degradation of Fatty Acids and Production of Biosurfactant as an Added Value, by a Bacterial Strain Pseudomonas aeruginosa DG2a Isolated from Aquaculture Wastewaters [Elektronische Daten] [Milva Pepi, Silvia Focardi, Arianna Lobianco, Diego Angelini, Francesca Borghini, Silvano Focardi] Aquaculture wastewaters, with oleic acid (C18:1 ω9) as the most representative contaminant fatty acid, were used as inoculum to perform enrichment cultures in mineral medium in the presence of oleic acid as the sole carbon and energy source, allowing isolation of four bacterial strains named DG1a, DG2a, DG1b and DG2b. 16S rRNA gene sequencing analysis assigned the four isolates to the species Pseudomonas aeruginosa. Among the isolates, P. aeruginosa strain DG2a showed degradation of fatty acids, including oleic acid (C18:1 ω9). The hydrophobicity features were investigated in strain DG2a, and a constitutive hydrophobicity in the bacterial cells was highlighted. The capability to produce biosurfactants by cells of the bacterial strain P. aeruginosa strain DG2a was evidenced both in the presence of oleic acid and of aquaculture wastewaters by revealing emulsifying activity, oil spreading tests, haemolytic and cetyltrimethylammonium bromide agar tests. Bacterial cultures containing raw biosurfactant were added to native wastewaters, showing a depletion of the oleic acid content. The use of the isolated bacterial strain P. aeruginosa strain DG2a and of the produced biosurfactant in bioremediation of aquaculture wastewaters is proposed, and the valorization of aquaculture wastewaters as raw material for biosurfactant production by using the isolate is moreover suggested. Springer Science+Business Media Dordrecht, 2013 Pseudomonas aeruginosa nationallicence Fatty acids nationallicence Biodegradation nationallicence Biosurfactants nationallicence Aquaculture waste nationallicence Bioremediation nationallicence Pepi Milva Department of Physical, Earth and Environmental Sciences, University of Siena, Via P.A. Mattioli, 4, 53100, Siena, Italy aut Focardi Silvia Department of Physical, Earth and Environmental Sciences, University of Siena, Via P.A. Mattioli, 4, 53100, Siena, Italy aut Lobianco Arianna Department of Physical, Earth and Environmental Sciences, University of Siena, Via P.A. Mattioli, 4, 53100, Siena, Italy aut Angelini Diego Department of Physical, Earth and Environmental Sciences, University of Siena, Via P.A. Mattioli, 4, 53100, Siena, Italy aut Borghini Francesca Department of Physical, Earth and Environmental Sciences, University of Siena, Via P.A. Mattioli, 4, 53100, Siena, Italy aut Focardi Silvano Department of Physical, Earth and Environmental Sciences, University of Siena, Via P.A. Mattioli, 4, 53100, Siena, Italy aut Water, Air, & Soil Pollution Springer Netherlands 224/11(2013-11-01), 1-11 0049-6979 224:11<1 2013 224 11270 https://doi.org/10.1007/s11270-013-1772-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11270-013-1772-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pepi Milva Department of Physical, Earth and Environmental Sciences, University of Siena, Via P.A. Mattioli, 4, 53100, Siena, Italy aut NATIONALLICENCE 700 1- Focardi Silvia Department of Physical, Earth and Environmental Sciences, University of Siena, Via P.A. Mattioli, 4, 53100, Siena, Italy aut NATIONALLICENCE 700 1- Lobianco Arianna Department of Physical, Earth and Environmental Sciences, University of Siena, Via P.A. Mattioli, 4, 53100, Siena, Italy aut NATIONALLICENCE 700 1- Angelini Diego Department of Physical, Earth and Environmental Sciences, University of Siena, Via P.A. Mattioli, 4, 53100, Siena, Italy aut NATIONALLICENCE 700 1- Borghini Francesca Department of Physical, Earth and Environmental Sciences, University of Siena, Via P.A. Mattioli, 4, 53100, Siena, Italy aut NATIONALLICENCE 700 1- Focardi Silvano Department of Physical, Earth and Environmental Sciences, University of Siena, Via P.A. Mattioli, 4, 53100, Siena, Italy aut NATIONALLICENCE 773 0- Water, Air, & Soil Pollution Springer Netherlands 224/11(2013-11-01), 1-11 0049-6979 224:11<1 2013 224 11270 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer