naa a22 4500 510752683 CHVBK 20180411083110.0 cr unu---uuuuu 180411e20131101xx s 000 0 eng 10.1007/s11270-013-1748-1 doi (NATIONALLICENCE)springer-10.1007/s11270-013-1748-1 Bacterial Diversity at Abandoned Uranium Mining and Milling Sites in Bulgaria as Revealed by 16S rRNA Genetic Diversity Study [Elektronische Daten] [Galina Radeva, Anelia Kenarova, Velina Bachvarova, Katrin Flemming, Ivan Popov, Dimitar Vassilev, Sonja Selenska-Pobell] Radionuclide and heavy metal contamination influences the composition and diversity of bacterial communities, thus adversely affecting their ecological role in impacted environments. Bacterial communities from uranium and heavy metal-contaminated soil environments and mine waste piles were analyzed using 16S rRNA gene retrieval. A total of 498 clones were selected, and their 16S rDNA amplicons were analyzed by restriction fragment length polymorphism, which suggested a total of 220 different phylotypes. The phylogenetic analysis revealed Proteobacteria, Acidobacteria, and Bacteroidetes as the most common bacterial taxa for the three sites of interest. Around 20-30% of the 16S rDNA sequences derived from soil environments were identified as Proteobacteria, which increased up to 76% (mostly Gammaproteobacteria) in bacterial communities inhabiting the mine waste pile. Acidobacteria, known to be common soil inhabitants, dominated in less contaminated environments, while Bacteroidetes were more abundant in highly contaminated environments regardless of the type of substratum (soil or excavated gravel material). Some of the sequences affiliated with Verrucomicrobia, Actinobacteria, Chloroflexi, Planctomycetes, and Candidate division OP10 were site specific. The relationship between the level of contamination and the rate of bacterial diversity was not linear; however, the bacterial diversity was generally higher in soil environments than in the mine waste pile. It was concluded that the diversity of the bacterial communities sampled was influenced by both the degree of uranium and heavy metal contamination and the site-specific conditions. Springer Science+Business Media Dordrecht, 2013 Bacterial diversity nationallicence 16S rRNA gene nationallicence Uranium and heavy metal contamination nationallicence Radeva Galina Institute of Resource Ecology, Helmholtz Centre Dresden-Rossendorf, Bautzner Landstr. 400, 01328, Dresden, Germany aut Kenarova Anelia Faculty of Biology, Sofia University, St. Kl. Ohridski, 8 Dragan Tzankov blvd., 1164, Sofia, Bulgaria aut Bachvarova Velina Institute of Molecular Biology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., bl. 21, 1113, Sofia, Bulgaria aut Flemming Katrin Institute of Resource Ecology, Helmholtz Centre Dresden-Rossendorf, Bautzner Landstr. 400, 01328, Dresden, Germany aut Popov Ivan Molecular Medicine Center, Medical University Sofia, 1 Georgy Sofiiski Str., 1431, Sofia, Bulgaria aut Vassilev Dimitar Agrobioinstitute, 8 Dragan Tsankov blvd., 1164, Sofia, Bulgaria aut Selenska-Pobell Sonja Institute of Resource Ecology, Helmholtz Centre Dresden-Rossendorf, Bautzner Landstr. 400, 01328, Dresden, Germany aut Water, Air, & Soil Pollution Springer Netherlands 224/11(2013-11-01), 1-14 0049-6979 224:11<1 2013 224 11270 https://doi.org/10.1007/s11270-013-1748-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11270-013-1748-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Radeva Galina Institute of Resource Ecology, Helmholtz Centre Dresden-Rossendorf, Bautzner Landstr. 400, 01328, Dresden, Germany aut NATIONALLICENCE 700 1- Kenarova Anelia Faculty of Biology, Sofia University, St. Kl. Ohridski, 8 Dragan Tzankov blvd., 1164, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Bachvarova Velina Institute of Molecular Biology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., bl. 21, 1113, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Flemming Katrin Institute of Resource Ecology, Helmholtz Centre Dresden-Rossendorf, Bautzner Landstr. 400, 01328, Dresden, Germany aut NATIONALLICENCE 700 1- Popov Ivan Molecular Medicine Center, Medical University Sofia, 1 Georgy Sofiiski Str., 1431, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Vassilev Dimitar Agrobioinstitute, 8 Dragan Tsankov blvd., 1164, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Selenska-Pobell Sonja Institute of Resource Ecology, Helmholtz Centre Dresden-Rossendorf, Bautzner Landstr. 400, 01328, Dresden, Germany aut NATIONALLICENCE 773 0- Water, Air, & Soil Pollution Springer Netherlands 224/11(2013-11-01), 1-14 0049-6979 224:11<1 2013 224 11270 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer