naa a22 4500 51075287X CHVBK 20180411083111.0 cr unu---uuuuu 180411e20131101xx s 000 0 eng 10.1007/s11270-013-1755-2 doi (NATIONALLICENCE)springer-10.1007/s11270-013-1755-2 Biotreatment of Melanoidin-Containing Distillery Spent Wash Effluent by Free and Immobilized Aspergillus oryzae MTCC 7691 [Elektronische Daten] [M. Chavan, N. Dandi, M. Kulkarni, A. Chaudhari] A total of three fungal isolates from samples collected at spent wash disposal area were screened for their ability to degrade melanoidin. Distillery molasses spent wash was decolorized, and its chemical oxygen demand (COD) was reduced in immobilized fungal bioreactor (IFB) in the absence of carbon and nitrogen source using fungal mycelia of Aspergillus oryzae MTCC 7691. Fungal mycelia immobilized on baggase packed in a glass column under a batch-wise mode (1) effected removal of 75.71 ± 0.12% color, 51.0 ± 0.13% biological oxygen demand (BOD), 86.19 ± 2.56% COD, and 49.0 ± 0.12% phenolic pigments of distillery spent wash up to 25days at 30°C, while free fungal mycelia resulted in removal of 63.1 ± 0.16% color, 27.74 ± 0.14% BOD, 76.21 ± 1.62% COD, and 37.32 ± 0.17% phenolic pigments of distillery spent wash using shake flask, (2) manganese peroxidase (MnP) activity was highest (1.55 ± 0.01Uml−1min−1) in immobilized fungi, followed by lignin peroxidase (0.65 ± 0.01Uml−1min−1) and laccase activity (0.9 ± 0.01CUml−1min−1), (3) accumulative MnP activity was highly correlated with (r = 0.9216) spent wash decolorization and (r = 0.7282) reduction of phenolic pigments, suggesting the presence of MnP activities in bioremediation of spent wash and (4) degradation of spent wash was confirmed by high-performance thin layer chromatography and gas chromatography-mass spectrometry analysis. Measurement of chlorophyll a content of Chlorella species cultivated on treated spent wash effluent obtained from immobilized fungal bioreactor was 5.16 ± 0.71μgml−1 compared with 1.306 ± 0.017μgml−1 obtained with untreated spent wash. Thus, this work may provide a reasonable alternative for cost-effective bioremediation of distillery spent wash using immobilized A. oryzae on baggase fibers. Springer Science+Business Media Dordrecht, 2013 Aspergillus oryzae MTCC 7691 nationallicence Bagasse nationallicence Bioreactor nationallicence Decolorization nationallicence GC-MS nationallicence Immobilization nationallicence Chavan M. Department of Biotechnology, Institute of Science, 431004, Aurangabad, MS, India aut Dandi N. Department of Microbiology, School of Life Sciences, North Maharashtra University, PO Box 80, 425001, Jalgaon, MS, India aut Kulkarni M. Division of Biochemistry, Department of Chemistry, University of Pune, 411007, Pune, MS, India aut Chaudhari A. Department of Microbiology, School of Life Sciences, North Maharashtra University, PO Box 80, 425001, Jalgaon, MS, India aut Water, Air, & Soil Pollution Springer Netherlands 224/11(2013-11-01), 1-10 0049-6979 224:11<1 2013 224 11270 https://doi.org/10.1007/s11270-013-1755-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11270-013-1755-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chavan M. Department of Biotechnology, Institute of Science, 431004, Aurangabad, MS, India aut NATIONALLICENCE 700 1- Dandi N. Department of Microbiology, School of Life Sciences, North Maharashtra University, PO Box 80, 425001, Jalgaon, MS, India aut NATIONALLICENCE 700 1- Kulkarni M. Division of Biochemistry, Department of Chemistry, University of Pune, 411007, Pune, MS, India aut NATIONALLICENCE 700 1- Chaudhari A. Department of Microbiology, School of Life Sciences, North Maharashtra University, PO Box 80, 425001, Jalgaon, MS, India aut NATIONALLICENCE 773 0- Water, Air, & Soil Pollution Springer Netherlands 224/11(2013-11-01), 1-10 0049-6979 224:11<1 2013 224 11270 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer