naa a22 4500 510753612 CHVBK 20180411083113.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s13131-013-0323-3 doi (NATIONALLICENCE)springer-10.1007/s13131-013-0323-3 Molecular diversity of Thermococcales isolated from Guaymas Basin hydrothermal vents [Elektronische Daten] [Lupeng Liu, Fengping Wang, Jun Xu, Xiang Xiao] Members of Thermococcales are organoheterotrophic hyperthermophilic anaerobes consisting of three genera, Thermococcus, Pyrococcus, and Palaeococcus, among which Thermococcus are the most frequently isolated. In this study, a variety of Thermococcales strains were isolated from theGuaymas Basin hydrothermal vents under different temperatures and pressures. Based on their 16S rRNA gene sequences, all the strains isolated from 85°C or 95°C enrichment cultures at ambient pressure were classified as Thermococcus, while strains isolated from 108°C-30MPa enrichment cultures belonged to Pyrococcus. The combination of high pressure and high temperature for enrichment was shown to be an efficientmethod for isolating Pyrococcus strains. Through selecting and analyzing with multiple molecular phylogeneticmarkers, the isolated Thermococcales strains were found quite diversified including several putative novel species. This demonstrates a vast genetic reservoir of Thermoccoccales in the deep-sea hydrothermal vents, and a fast evolving of the Thermococcales species adapting to the fast changing environment. The Chinese Society of Oceanography and Springer-Verlag Berlin Heidelberg, 2013 hydrothermal vents nationallicence Thermococcales nationallicence Guaymas Basin nationallicence Liu Lupeng College of Marine Life Sciences, Ocean University of China, 266003, Qingdao, China aut Wang Fengping State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, China aut Xu Jun State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, China aut Xiao Xiang State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, China aut Acta Oceanologica Sinica The Chinese Society of Oceanography 32/6(2013-06-01), 75-81 0253-505X 32:6<75 2013 32 13131 https://doi.org/10.1007/s13131-013-0323-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13131-013-0323-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Lupeng College of Marine Life Sciences, Ocean University of China, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Wang Fengping State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, China aut NATIONALLICENCE 700 1- Xu Jun State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, China aut NATIONALLICENCE 700 1- Xiao Xiang State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, China aut NATIONALLICENCE 773 0- Acta Oceanologica Sinica The Chinese Society of Oceanography 32/6(2013-06-01), 75-81 0253-505X 32:6<75 2013 32 13131 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer