naa a22 4500 510753647 CHVBK 20180411083113.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s13131-013-0324-2 doi (NATIONALLICENCE)springer-10.1007/s13131-013-0324-2 Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in Skeletonema costatum [Elektronische Daten] [Yun Liu, Xiuxian Song, Xiaotian Han, Zhiming Yu] To verify the feasibility of high-affinity nitrate transporter gene (Nrt2) as an indicator of nitrogen status, changes in the transcript levels of transcripts associated with phosphate starvation and different nitrate concentrations were studied using real-time quantitative reverse-transcription PCR (QRT-PCR) technology in batch cultures of Skeletonema costatum. The results show that compared with P-replete condition, P starvation could reduce the Nrt2 transcript levels apparently. Nrt2 transcript levels had a significant negative linear correlation with nitrate concentrations below 40 µmol/L. The results of 48 h short-term incubation experiment under different nitrate concentrations confirmed this correlation, and the following regression equation is built: y = −3.305x +98.95, R 2 = 0.988, where x represents nitrate concentrations (<40 µmol/L) and y represents the Nrt2 transcript levels. The Chinese Society of Oceanography and Springer-Verlag Berlin Heidelberg, 2013 high-affinity nitrate transporter gene nationallicence phosphate starvation nationallicence nitrate concentrations nationallicence real-time PCR nationallicence Skeletonema costatum nationallicence Liu Yun Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut Song Xiuxian Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut Han Xiaotian Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut Yu Zhiming Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut Acta Oceanologica Sinica The Chinese Society of Oceanography 32/6(2013-06-01), 82-88 0253-505X 32:6<82 2013 32 13131 https://doi.org/10.1007/s13131-013-0324-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13131-013-0324-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Yun Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut NATIONALLICENCE 700 1- Song Xiuxian Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut NATIONALLICENCE 700 1- Han Xiaotian Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut NATIONALLICENCE 700 1- Yu Zhiming Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut NATIONALLICENCE 773 0- Acta Oceanologica Sinica The Chinese Society of Oceanography 32/6(2013-06-01), 82-88 0253-505X 32:6<82 2013 32 13131 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer