naa a22 4500 510753663 CHVBK 20180411083113.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s13131-013-0271-y doi (NATIONALLICENCE)springer-10.1007/s13131-013-0271-y Phylogenetic diversity of planktonic bacteria in the Chukchi Borderland region in summer [Elektronische Daten] [Yinxin Zeng, Yong Yu, Huirong Li, Jianfeng He, Sang Lee, Kun Sun] Planktonic bacteria are abundant in the Chukchi Borderland region. However, little is known about their diversity and the roles of various bacteria in the ocean. Seawater samples were collected from two stations K2S and K4S where sea ice was melting obviously. The analysis of water samples with fluorescence in situ hybridization (FISH) showed that DMSP-degrading bacteria accounted for 13% of the total bacteria at the station K2S. No aerobic anoxygenic phototrophic (AAP) bacteria were detected in both samples. The bacterial communities were characterized by two 16S rRNA gene clone libraries. Sequences fell into four major lineages of the domain Bacteria, including Proteobacteria (Alpha, Beta and Gamma subclasses), Bacteroidetes, Actinobacteria and Firmicutes. No significant difference was found between the two clone libraries. SAR11 and Rhodobacteraceae clades of Alphaproteobacteria and Pseudoalteromonas of Gammaproteobacteria constituted three dominant fractions in the clone libraries. A total of 191 heterotrophic bacterial strains were isolated and 76% showed extracellular proteolytic activity. Phylogenetic analysis reveals that the isolates fell into Gammaproteobacteria, Bacteroidetes, Actinobacteria and Firmicutes. The most common genus in both the bacterial isolates and protease-producing bacteria was Pseudoalteromonas. UniFracdata showed suggestive differences in bacterial communities between the Chukchi Borderland and the northern Bering Sea. The Chinese Society of Oceanography and Springer-Verlag Berlin Heidelberg, 2013 diversity nationallicence planktonic bacteria nationallicence 16S rRNA gene clone library nationallicence Chukchi Borderland nationallicence Zeng Yinxin Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, 200136, Shanghai, China aut Yu Yong Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, 200136, Shanghai, China aut Li Huirong Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, 200136, Shanghai, China aut He Jianfeng Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, 200136, Shanghai, China aut Lee Sang Department of Oceanography, Pusan National University, 609735, Busan, Korea aut Sun Kun Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, 200136, Shanghai, China aut Acta Oceanologica Sinica The Chinese Society of Oceanography 32/6(2013-06-01), 66-74 0253-505X 32:6<66 2013 32 13131 https://doi.org/10.1007/s13131-013-0271-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13131-013-0271-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zeng Yinxin Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, 200136, Shanghai, China aut NATIONALLICENCE 700 1- Yu Yong Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, 200136, Shanghai, China aut NATIONALLICENCE 700 1- Li Huirong Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, 200136, Shanghai, China aut NATIONALLICENCE 700 1- He Jianfeng Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, 200136, Shanghai, China aut NATIONALLICENCE 700 1- Lee Sang Department of Oceanography, Pusan National University, 609735, Busan, Korea aut NATIONALLICENCE 700 1- Sun Kun Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, 200136, Shanghai, China aut NATIONALLICENCE 773 0- Acta Oceanologica Sinica The Chinese Society of Oceanography 32/6(2013-06-01), 66-74 0253-505X 32:6<66 2013 32 13131 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer