naa a22 4500 510754422 CHVBK 20180411083117.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s13131-013-0341-1 doi (NATIONALLICENCE)springer-10.1007/s13131-013-0341-1 Disruption of chemotaxis-related genes affects multiple cellular processes and the virulence of pathogenic Vibrio harveyi [Elektronische Daten] [Yingxue Qin, Qingpi Yan, Yongquan Su, Haiping Li, Wenzheng Zou] Chemotactic motility is involved in the virulence of many pathogenic bacteria. In order to understand the role of chemotactic motility of Vibrio harveyi in cellular processes and virulence, mini-Tn10/Kan transposon-induced mutants with deficient chemotactic motility were constructed, screened, and identified. Sequence analysis revealed that the 465-bp fragment (Gen Bank accession number HM630274) flanking the transposon insertion site in mutant TS-CM1 had the highest identity (96.9%) with a hypothetical protein gene of V. harveyi ATCC BAA-1116 and the second-highest identity (91.8%) with the pgk gene of V. parahaemolyticus RIMD 2210633. In another mutant, TS-CM2, 356 bp of transposon-flanking sequence (Gen Bank accession number HM630275) also showed the highest identity (94.6%) with a hypothetical protein gene of V. harveyi ATCC BAA-1116 and the second-highest identity (92.4%) with the flaB gene of V. alginolyticus HY9901. Studies on virulence-related biological characteristics such as growth, motility, adhesion, and infectivity of themutants showed that disruption of either the flagellin gene or energy metabolism gene led to subsequent loss of chemotactic motility and changes in growth, motility, adhesion, and virulence of the pathogenic V. harveyi. Hence, the flagellin gene and crucial energy metabolism gene played an important role in the chemotactic motility of V. harveyi. The Chinese Society of Oceanography and Springer-Verlag Berlin Heidelberg, 2013 chemotactic motility nationallicence Vibrio harveyi nationallicence transposon nationallicence virulence nationallicence Qin Yingxue Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, 361021, Xiamen, China aut Yan Qingpi Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, 361021, Xiamen, China aut Su Yongquan College of Ocean and Earth Science, Xiamen University, 361005, Xiamen, China aut Li Haiping Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, 361021, Xiamen, China aut Zou Wenzheng Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, 361021, Xiamen, China aut Acta Oceanologica Sinica Springer Berlin Heidelberg 32/8(2013-08-01), 55-60 0253-505X 32:8<55 2013 32 13131 https://doi.org/10.1007/s13131-013-0341-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13131-013-0341-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Qin Yingxue Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, 361021, Xiamen, China aut NATIONALLICENCE 700 1- Yan Qingpi Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, 361021, Xiamen, China aut NATIONALLICENCE 700 1- Su Yongquan College of Ocean and Earth Science, Xiamen University, 361005, Xiamen, China aut NATIONALLICENCE 700 1- Li Haiping Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, 361021, Xiamen, China aut NATIONALLICENCE 700 1- Zou Wenzheng Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, 361021, Xiamen, China aut NATIONALLICENCE 773 0- Acta Oceanologica Sinica Springer Berlin Heidelberg 32/8(2013-08-01), 55-60 0253-505X 32:8<55 2013 32 13131 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer