naa a22 4500 510754465 CHVBK 20180411083117.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s13131-013-0339-8 doi (NATIONALLICENCE)springer-10.1007/s13131-013-0339-8 Characterization of the cyp19a1a gene from a BAC sequence in half-smooth tongue sole ( Cynoglossus semilaevis ) and analysis of its conservation among teleosts [Elektronische Daten] [Changwei Shao, Geng Liu, Shanshan Liu, Changlin Liu, Songlin Chen] The cyp19a1a gene encodes an aromatase that plays a key role in sex differentiation of the gonad. The first bacterial artificial chromosome (BAC) sequence of half-smooth tongue sole (Cynoglossus semilaevis) containing the intact cyp19a1a gene was reported and the conservation and synteny of the cyp19a1a gene among teleosts were analyzed in the study. The BAC is 107 367 bp in size, with an overall guanine-cytosine (GC) content of 43.44%, and contains 4.38% transposable elements. Nine genes were predicted, including seven functional genes and two hypothetical genes. The cyp19a1a gene of all tested teleosts has nine exons and eight introns, and potential binding sites flanking the transcriptional start site are conserved. The expression pattern among teleosts is also similar during ovarian differentiation. Synteny analysis revealed a conserved gene cluster PKH4B-SL9A5-FHOD3-CEBPG-CEBPA among teleosts. These findings suggest that, among teleosts, cyp19a1a genes not only have similar genomic structures, but also have conserved functions. The genomic environment of cyp19a1a in tongue sole is not universal in teleosts, reflecting the particular evolution of tongue sole cyp19a1a after it diverged from the other teleosts. The Chinese Society of Oceanography and Springer-Verlag Berlin Heidelberg, 2013 BAC nationallicence cyp19a1a nationallicence conservation nationallicence gene synteny nationallicence half-smooth tongue sole nationallicence Shao Changwei Ocean University of China, 266071, Qingdao, China aut Liu Geng BGI-Shenzhen, 518000, Shenzhen, China aut Liu Shanshan Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 266071, Qingdao, China aut Liu Changlin Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 266071, Qingdao, China aut Chen Songlin Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 266071, Qingdao, China aut Acta Oceanologica Sinica Springer Berlin Heidelberg 32/8(2013-08-01), 35-43 0253-505X 32:8<35 2013 32 13131 https://doi.org/10.1007/s13131-013-0339-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13131-013-0339-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shao Changwei Ocean University of China, 266071, Qingdao, China aut NATIONALLICENCE 700 1- Liu Geng BGI-Shenzhen, 518000, Shenzhen, China aut NATIONALLICENCE 700 1- Liu Shanshan Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 266071, Qingdao, China aut NATIONALLICENCE 700 1- Liu Changlin Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 266071, Qingdao, China aut NATIONALLICENCE 700 1- Chen Songlin Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, 266071, Qingdao, China aut NATIONALLICENCE 773 0- Acta Oceanologica Sinica Springer Berlin Heidelberg 32/8(2013-08-01), 35-43 0253-505X 32:8<35 2013 32 13131 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer