naa a22 4500 510754511 CHVBK 20180411083117.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s13131-013-0343-z doi (NATIONALLICENCE)springer-10.1007/s13131-013-0343-z Generation and analysis of expressed sequence tags fromthe salt-tolerant eelgrass species, Zostera marina [Elektronische Daten] [Fanna Kong, Yang Zhou, Peipei Sun, Limin Liu, Yunxiang Mao] Zostera marina, a monocotyledonous angiosperm, is one of the most important seagrass species. To investigate the salt-tolerance mechanism and discover salt-tolerant genes in Z. marina, a cDNA library was constructed. Single-pass sequencing of the 5′ ends of 4 081 clones yielded 4 002 high quality expressed sequence tags (ESTs), which were assembled into 241 contigs and 1 673 singletons, representing 1 914 unigenes. The average length of the ESTs was 582 bp, with sizes ranging from 100-1 500 bp. Basic Local Alignment Search Tool (BLASTX) analysis revealed that 1 664 unigenes had significant homology to known genes in the National Center for Biotechnology Information (NCBI) non-redundant (nr) database (E-value⩽5-10). Among them, the two most abundant genes encoded metallothionein (157 ESTs) and chlorophyll a/b-binding protein (38 ESTs), accounting for 7.1% and 1.7% of the total ESTs, respectively. Using Kyoto Encyclopedia of Genes and Genomes (KEGG), 1 462 unigenes were assigned to 1 161 pathways (E-value⩽5-10). A total of 938 unigenes were assigned Gene Ontology (GO) terms based on the GO hierarchy analysis, and InterProScan searches recognized 1 003 InterPro families. Three genes for metallothionein in Z. marina that belonged to Class II was identified. Results of this study will improve understanding of the molecular mechanisms of saline tolerance in Z. marina. The Chinese Society of Oceanography and Springer-Verlag Berlin Heidelberg, 2013 expressed sequence tags (ESTs) nationallicence gene function nationallicence metallothionein nationallicence salt-tolerant genes nationallicence Zostera marina nationallicence Kong Fanna College of Marine Life Sciences, Ocean University of China, 266003, Qingdao, China aut Zhou Yang College of Marine Life Sciences, Ocean University of China, 266003, Qingdao, China aut Sun Peipei College of Marine Life Sciences, Ocean University of China, 266003, Qingdao, China aut Liu Limin College of Marine Life Sciences, Ocean University of China, 266003, Qingdao, China aut Mao Yunxiang College of Marine Life Sciences, Ocean University of China, 266003, Qingdao, China aut Acta Oceanologica Sinica Springer Berlin Heidelberg 32/8(2013-08-01), 68-78 0253-505X 32:8<68 2013 32 13131 https://doi.org/10.1007/s13131-013-0343-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13131-013-0343-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kong Fanna College of Marine Life Sciences, Ocean University of China, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Zhou Yang College of Marine Life Sciences, Ocean University of China, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Sun Peipei College of Marine Life Sciences, Ocean University of China, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Liu Limin College of Marine Life Sciences, Ocean University of China, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Mao Yunxiang College of Marine Life Sciences, Ocean University of China, 266003, Qingdao, China aut NATIONALLICENCE 773 0- Acta Oceanologica Sinica Springer Berlin Heidelberg 32/8(2013-08-01), 68-78 0253-505X 32:8<68 2013 32 13131 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer