naa a22 4500 510754651 CHVBK 20180411083118.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s13131-013-0276-6 doi (NATIONALLICENCE)springer-10.1007/s13131-013-0276-6 Microbial community structure and diversity in deep-sea hydrothermal vent sediments along the Eastern Lau Spreading Centre [Elektronische Daten] [Manman Wei, Rubing Zhang, Yuguang Wang, Houguo Ji, Jia Zheng, Xinhua Chen, Hongbo Zhou] The aim of this study is to investigate microbial structures and diversities in five active hydrothermal fields' sediments along the Eastern Lau Spreading Centre (ELSC) in the Lau Basin (southwest Pacific). Microbial communities were surveyed by denatured gradient gel electrophoresis (DGGE) and clone library analysis of 16S rRNA genes. The differences in microbial community structures among sediment samples from the five deep-sea hydrothermal sites were revealed by DGGE profiles. Cluster analysis of DGGE profiles separated the five hydrothermal samples into two groups. Four different 16S rRNA gene clone libraries, representing two selected hydrothermal samples (19-4TVG8 and 19-4TVG11), were constructed. Twenty-three and 32 phylotypes were identified from166 and 160 bacterial clones respectively, including Proteobacteria, Bacteroidetes, Firmicutes, Nitrospirae and Planctomycetes. The phylum Proteobacteria is dominant in both bacterial libraries with a predominance of Gamma-Proteobacteria. A total of 31 and 25 phylotypes were obtained from 160 and 130 archaeal clones respectively, including Miscellaneous Crenarchaeotic Group, Marine Group I and III, Marine Benthic Group E, Terrestrial Hot Spring Crenarchaeota and Deep-sea Hydrothermal Vent Euryarchaeota. These results show a variety of clones related to those involved in sulfur cycling, suggesting that the cycling and utilization of sulfur compounds may extensively occur in the Lau Basin deep-sea hydrothermal ecosystem. The Chinese Society of Oceanography and Springer-Verlag Berlin Heidelberg, 2013 microbial diversity nationallicence deep-sea hydrothermal vent nationallicence DGGE nationallicence 16S rRNA gene clone libraries nationallicence Wei Manman School of Minerals Processing and Bioengineering, Central South University, 410083, Changsha, China aut Zhang Rubing Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China aut Wang Yuguang School of Minerals Processing and Bioengineering, Central South University, 410083, Changsha, China aut Ji Houguo School of Minerals Processing and Bioengineering, Central South University, 410083, Changsha, China aut Zheng Jia School of Minerals Processing and Bioengineering, Central South University, 410083, Changsha, China aut Chen Xinhua Third Institute of Oceanography State, Oceanic Administration, 361005, Xiamen, China aut Zhou Hongbo School of Minerals Processing and Bioengineering, Central South University, 410083, Changsha, China aut Acta Oceanologica Sinica The Chinese Society of Oceanography 32/2(2013-02-01), 42-51 0253-505X 32:2<42 2013 32 13131 https://doi.org/10.1007/s13131-013-0276-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13131-013-0276-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wei Manman School of Minerals Processing and Bioengineering, Central South University, 410083, Changsha, China aut NATIONALLICENCE 700 1- Zhang Rubing Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, China aut NATIONALLICENCE 700 1- Wang Yuguang School of Minerals Processing and Bioengineering, Central South University, 410083, Changsha, China aut NATIONALLICENCE 700 1- Ji Houguo School of Minerals Processing and Bioengineering, Central South University, 410083, Changsha, China aut NATIONALLICENCE 700 1- Zheng Jia School of Minerals Processing and Bioengineering, Central South University, 410083, Changsha, China aut NATIONALLICENCE 700 1- Chen Xinhua Third Institute of Oceanography State, Oceanic Administration, 361005, Xiamen, China aut NATIONALLICENCE 700 1- Zhou Hongbo School of Minerals Processing and Bioengineering, Central South University, 410083, Changsha, China aut NATIONALLICENCE 773 0- Acta Oceanologica Sinica The Chinese Society of Oceanography 32/2(2013-02-01), 42-51 0253-505X 32:2<42 2013 32 13131 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer