naa a22 4500 510754724 CHVBK 20180411083118.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s13131-013-0261-0 doi (NATIONALLICENCE)springer-10.1007/s13131-013-0261-0 Enhanced resistance of marine shrimp Exopalamon carincauda Holthuis to WSSV by injecting live VP28-recombinant bacteria [Elektronische Daten] [Yumiao Sun, Fuhua Li, Yanhong Chi, Jianhai Xiang] Recombinant bacteria secreting the transmembrane-truncated region of white spot syndrome virus (WSSV) envelope protein VP28 named DhpVB were constructed, using live Escherichia coli as a deliverer and a constitutive secretory expression plasmid as the expression vector. With the ability to deliver recombinant protein products outside, DhpVB couldmake VP28 directly interact with the shrimp when injected into shrimp. In order to test the protection potential, live DhpVB were injected into the marine shrimp Exopalamon carincauda Holthuis for three times and WSSV challenge was performed twice simultaneously at the last two injections of the bacteria. The results showed that DhpVB displayed statistically significant advantage over bacteria without VP28 after the second challenge with an average RI (Resistance index) of 0.67±0.08, compared with 0.41±0.09 of bacteria without VP28 (P <0.05). The data suggested that a quasi-immune response may exist in E. carincauda and live bacteria carrying VP28 could enhance the shrimp resistance against WSSV. The Chinese Society of Oceanography and Springer-Verlag Berlin Heidelberg, 2013 WSSV nationallicence VP28 nationallicence Exopalamon carincauda nationallicence live bacterial deliver nationallicence Sun Yumiao Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut Li Fuhua Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut Chi Yanhong Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut Xiang Jianhai Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut Acta Oceanologica Sinica The Chinese Society of Oceanography 32/2(2013-02-01), 52-58 0253-505X 32:2<52 2013 32 13131 https://doi.org/10.1007/s13131-013-0261-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13131-013-0261-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sun Yumiao Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut NATIONALLICENCE 700 1- Li Fuhua Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut NATIONALLICENCE 700 1- Chi Yanhong Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut NATIONALLICENCE 700 1- Xiang Jianhai Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut NATIONALLICENCE 773 0- Acta Oceanologica Sinica The Chinese Society of Oceanography 32/2(2013-02-01), 52-58 0253-505X 32:2<52 2013 32 13131 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer